Fusarium head blight(FHB)caused by Fusarium graminearum species complex (FGSC) is one of the most important diseases around the world. Deoxynivalenol (DON) is a type of mycotoxin produced by FGSC when infecting cereal crops. It is a serious threat to the health of both humans and livestock. Trehalose-6-phosphate phosphatase (TPP), a conserved metabolic enzyme found in many plants and pathogens, catalyzes the formation of trehalose. N-(phenylthio) phthalimide (NPP) has been reported to inhibit the normal growth of nematodes by inhibiting the activity of TPP, but this inhibitor of nematodes has not previously been tested against F. graminearum. In this study, we found that TPP in F. graminearum (FgTPP) had similar secondary structures and conserved cysteine (Cys356) to nematodes by means of bioinformatics. At the same time, the sensitivity of F. graminearum strains to NPP was determined. NPP exhibited a better inhibitory effect on conidia germination than mycelial growth. In addition, the effects of NPP on DON biosynthesis and trehalose biosynthesis pathway in PH-1 were also determined. We found that NPP decreased DON production, trehalose content, glucose content and TPP enzyme activity but increased trehalose-6-phosphate content and trehalose-6-phosphate synthase (TPS) enzyme activity. Moreover, the expression of TRI1, TRI4, TRI5, TRI6, and TPP genes were downregulated, on the contrary, the TPS gene was upregulated. Finally, in order to further determine the control ability of NPP on DON production in the field, we conducted a series of field experiments, and found that NPP could effectively reduce the DON content in wheat grain and had a general control effect on FHB. In conclusion, the research in this study will provide important theoretical basis for controlling FHB caused by F. graminearum and reducing DON production in the field.

由禾谷镰刀菌物种复合体（FGSC ）引起的镰刀菌枯萎病（FHB）是世界上最重要的病害之一。脱氧雪腐镰刀菌烯醇 (DON) 是 FGSC 在感染谷类作物时产生的一种霉菌毒素。它严重威胁着人类和牲畜的健康。6-磷酸海藻糖磷酸酶 ( TPP ) 是一种在许多植物和病原体中发现的保守代谢酶，可催化海藻糖的形成。据报道，N-（苯硫基）邻苯二甲酰亚胺 (NPP) 通过抑制TPP的活性来抑制线虫的正常生长，但这种线虫抑制剂之前尚未针对禾谷镰刀菌进行过测试。在本研究中，我们发现TPP在禾谷镰刀菌（FG TPP）利用生物信息学的方法具有类似的二级结构和保守的半胱氨酸（Cys356）至线虫。同时，测定了禾谷镰刀菌菌株对 NPP的敏感性。NPP 对分生孢子萌发的抑制作用优于菌丝生长。此外，还测定了NPP对PH-1中DON生物合成和海藻糖生物合成途径的影响。我们发现NPP降低了DON的产生、海藻糖含量、葡萄糖含量和TPP酶活性，但增加了6-磷酸海藻糖含量和6-磷酸海藻糖合酶( TPS )酶活性。此外，TR I 1的表达，TRI 4、TR I 5、TR I 6和TPP基因被下调，相反，TPS基因被上调。最后，为进一步确定NPP对田间DON产生的控制能力，我们进行了一系列田间试验，发现NPP能有效降低小麦籽粒DON含量，对FHB有一般防治效果。综上所述，本研究将为控制禾谷镰刀菌引起的FHB ，减少田间DON的产生提供重要的理论依据。