Metabolome analysis in micro physiological models is a challenge due to the low volume of the cell culture medium (CCM). Here, we report a LC-MS-based untargeted metabolomics protocol for the detection of hepatocyte extracellular metabolites from micro-scale samples of CCM. Using a single LC-MS method we have detected 57 metabolites of which 27 showed >2-fold shifts after 72-hour incubation. We demonstrate that micro-scale CCM samples can be used for modelling micro-physiological temporal dynamics in metabolite intensities.

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中文翻译：

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用于分析来自肝细胞的微尺度细胞外代谢物的非靶向 LC-MS 代谢组学

由于细胞培养基 (CCM) 的体积小，微生理模型中的代谢组分析是一项挑战。在这里，我们报告了一种基于 LC-MS 的非靶向代谢组学协议，用于从 CCM 的微型样本中检测肝细胞细胞外代谢物。使用单一 LC-MS 方法，我们检测到 57 种代谢物，其中 27 种在孵育 72 小时后表现出 >2 倍的变化。我们证明微尺度 CCM 样品可用于模拟代谢物强度的微生理时间动态。

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