Liposcelis entomophila (Psocodea: Liposcelididae) is one of the most economically important booklice in the world. This species damages stored grain and represents an allergenic health risk to humans. Because of the small size of booklice, they are difficult to identify using morphological characteristics. Traditional molecular identification, such as DNA barcoding and qRT-PCR, requires specialized equipment and takes a long time, which is not appropriate for practical usage by plant health inspectors at ports. Therefore, for storage psocid identification, we suggested a simple, rapid and relatively new technique known as loop-mediated isothermal amplification (LAMP). In our study, a new rapid molecular identification method using the LAMP technique based on the mitochondrial gene cytochrome c oxidase I (COI) was developed for L. entomophila with high specificity and efficiency. Evident amplification results could be presented if the DNA concentration was 0.001 ng/μL. Visual detection was also possible with our LAMP primers using hydroxy naphthol blue (HNB), relying on the clear change in color from violet to sky blue after amplification. This method successfully determined unknown species that were newly collected from grain stores and may facilitate L. entomophila identification in plant quarantine and stored product protection.

Liposcelis entomophila (Psocodea: Liposcelididae) 是世界上经济上最重要的书虱之一。该物种会破坏储存的谷物，并对人类造成过敏性健康风险。由于书虱的尺寸小，使用形态特征很难识别它们。传统的分子鉴定，如DNA条形码、qRT-PCR等，需要专门的设备，耗时长，不适合港口植物卫生检查人员实际使用。因此，对于存储 psocid 鉴定，我们提出了一种简单、快速且相对较新的技术，称为环介导等温扩增 (LAMP)。在我们的研究中，一种基于线粒体基因细胞色素c的 LAMP 技术快速分子鉴定新方法氧化酶 I (COI) 是为L. entomophila开发的，具有高特异性和效率。如果 DNA 浓度为 0.001 ng/μL，则可以呈现明显的扩增结果。我们的 LAMP 引物也可以使用羟基萘酚蓝 (HNB) 进行视觉检测，这依赖于扩增后颜色从紫色到天蓝色的明显变化。该方法成功地确定了从粮食仓库新收集的未知物种，可能有助于植物检疫和储藏产品保护中的L. entomophila鉴定。