The effects of both Tl⁺ and thiol reagents were studied on the content of the inner membrane free SH-groups, detected with Ellman reagent, and the inner membrane potential as well as swelling and respiration of succinate-energized rat liver mitochondria in medium containing TlNO₃ and KNO₃. These effects resulted in a rise in swelling and a decrease in the content, the potential, and mitochondrial respiration in 3 and 2,4-dinitrophenol-uncoupled states. A maximal effect was seen when phenylarsine oxide reacting with thiol groups recessed into the hydrophobic regions of the membrane. Compared with phenylarsine oxide, the effective concentrations of other reagents were approximately one order of magnitude higher in experiments with mersalyl and 4,4′-diisothiocyanostilbene-2,2′-disulfonate, and two orders of magnitude higher in experiments with tert-butyl hydroperoxide and diamide. The above effects of Tl⁺ and the thiol reagents became even more pronounced with calcium overload of mitochondria. However, the effects were suppressed by inhibitors of the mitochondrial permeability transition pore (cyclosporine A, ADP, and n-ethylmaleimide). These findings suggest that opening of the pore induced by Tl⁺ in the inner membrane can be dependent on the conformation state of the adenine nucleotide translocase, which depends on the activity of its thiol groups.

研究了Tl ⁺和硫醇试剂对内膜游离SH-基团含量的影响，用Ellman试剂检测，内膜电位以及琥珀酸激活的大鼠肝线粒体在含有TlNO的培养基中的膨胀和呼吸作用。₃和硝酸钾₃. 这些影响导致 3 和 2,4-二硝基苯酚未偶联状态下肿胀增加和含量、电位和线粒体呼吸减少。当与硫醇基团反应的氧化苯胂沉入膜的疏水区域时，可以看到最大的效果。与氧化苯胂相比，其他试剂的有效浓度在使用 mersalyl 和 4,4'-diisothiocyanostilbene-2,2'-disulfonate 的实验中高出大约一个数量级，在使用叔丁基过氧化氢的实验中高出两个数量级和二酰胺。^{Tl +}的上述效果随着线粒体钙超载，硫醇试剂变得更加明显。然而，线粒体通透性转换孔的抑制剂（环孢菌素 A、ADP 和n-乙基马来酰亚胺）抑制了这些作用。这些发现表明，由 Tl ⁺诱导的内膜孔打开可能取决于腺嘌呤核苷酸转位酶的构象状态，这取决于其硫醇基团的活性。