Objective Pseudomonas aeruginosa is the most important cause of lung infection among cystic fibrosis (CF) patients, and to reduce the severity of the infection, facility-specific cumulative antibiograms could help clinicians in empirical treatment.

Methods Respiratory samples of CF patients between January 2015 and December 2018 were scanned through Laboratory Operating System retrospectively. Demographical data of patients, culture results, and antibiotic susceptibilities are recorded using Microsoft Excel 2010. Cumulative antibiogram data were obtained according to the CLSI M39A4 document.

Results The number of registered patients has increased in 4 years from 154 to 253. The mean age of patients varied from 9 to 11.7 (range, 2–42). The ratio of patients with a positive culture for P. aeruginosa increased from 32 to 40%, and the mean patients' age decreased from 16.6 to 11.1 (p <0.05). A total number of 4,146 respiratory samples were analyzed. Sputum samples consisted of 42.5% (n: 1,767) of the samples with a 58.4% isolation rate of P. aeruginosa (n: 1,034). A notable increase of resistance was seen almost all antimicrobials tested by years. The ratio of multidrug-resistant (MDR) P. aeruginosa was 4.1, 10.2, 4.5, and 8.6% in 2015, 2016, 2017, and 2018.

Conclusion Antimicrobial resistance is a challenging problem in CF patients, and surveillance should be done regularly.

目的 铜绿假单胞菌是囊性纤维化 (CF) 患者肺部感染的最重要原因，为了降低感染的严重程度，针对特定机构的累积抗菌谱可以帮助临床医生进行经验性治疗。

方法 通过实验室操作系统对2015年1月至2018年12月期间CF患者的呼吸道样本进行回顾性扫描。使用 Microsoft Excel 2010 记录患者的人口统计学数据、培养结果和抗生素敏感性。根据 CLSI M39A4 文件获得累积抗菌谱数据。

结果 登记的患者人数在 4 年内从 154 人增加到 253 人。患者的平均年龄从 9 岁到 11.7 岁（范围，2-42 岁）不等。铜绿假单胞菌培养阳性的患者比例从 32% 增加到 40%，患者的平均年龄从 16.6 岁下降到 11.1 岁（p <0.05）。共分析了 4,146 个呼吸道样本。痰样本由 42.5% ( n : 1,767) 的样本组成，铜绿假单胞菌的分离率为 58.4% ( n : 1,034)。几乎所有经过多年测试的抗微生物药物都出现了耐药性的显着增加。2015、2016、2017和2018年耐多药（MDR）铜绿假单胞菌的比例分别为4.1、10.2、4.5和8.6%。

结论 抗生素耐药性是 CF 患者的一个具有挑战性的问题，应定期进行监测。