Purpose: The aim of this study was to optimize the technique of performing vitrectomy-assisted biopsy of intraocular tumors by comparing the cytohistological findings in specimens obtained with different vitrectomy probes and cut rates. Methods: Vitrectomy-assisted biopsies were taken from a fresh porcine liver. For each sampling, the vacuum level was 300 mm Hg. The following parameters were compared; cut rate (60, 600 and 6,000 cuts per minute [cpm]), probe type (standard and two-dimensional cutting [TDC]), and probe diameter (23-gauge and 25-gauge). The specimens were assessed by automated whole-slide imaging analysis and conventional light microscopy. Results: Seventy-two biopsies were analyzed for the number of hepatocytes, total area of tissue fragments, and total stained area of each microscope slide. For all probe types, these parameters were significantly and positively correlated with the cut rate. TDC probes led to significantly higher scores than those of standard probes, independent of the cut rate. There were no significant differences in results when using 23-gauge or 25-gauge standard probes. Light microscopic examination demonstrated well-preserved cells sufficient for cytohistological analyses in all investigated cases. Conclusions: The higher the cut rate, the larger is the amount of aspirated cellular material. There were no significant differences between 23-gauge and 25-gauge biopsies. Cut rates up to 6,000 cpm did not adversely affect the cytohistological features of the samples.
Ocul Oncol Pathol

中文翻译：

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玻璃体切除术辅助活检：关于切割率和探针尺寸影响的体外研究

目的：本研究的目的是通过比较不同玻璃体切割探针和切割率获得的标本的细胞组织学结果，优化对眼内肿瘤进行玻璃体切割辅助活检的技术。方法：玻璃体切割辅助活检取自新鲜猪肝。对于每次采样，真空度为 300 mm Hg。比较了以下参数；切割速率（每分钟 60、600 和 6,000 次切割 [cpm]）、探头类型（标准和二维切割 [TDC]）和探头直径（23 号和 25 号）。通过自动全载玻片成像分析和常规光学显微镜对标本进行评估。结果：分析了 72 个活组织检查的肝细胞数量、组织碎片的总面积和每个显微镜载玻片的总染色面积。对于所有探针类型，这些参数与切割率显着正相关。TDC 探针的得分明显高于标准探针，与切割率无关。使用 23 号或 25 号标准探头时，结果没有显着差异。光学显微镜检查显示保存完好的细胞足以在所有调查的病例中进行细胞组织学分析。结论：切割率越高，吸入的细胞物质的量就越大。23 号和 25 号活检之间没有显着差异。高达 6,000 cpm 的切割率不会对样品的细胞组织学特征产生不利影响。
眼肿瘤病理