Introduction: This study investigated the clinical values of miR-27a-3p for pulpitis patients, and its association with TLR4. Methods: Sixty-six patients with pulpitis and 34 cases without pulpitis were recruited; the pulp tissue and serum samples were collected from each participant. Real-time polymerase chain reaction was used for measurement of gene expression levels. The diagnosis values were assessed by the receiver operating characteristic curve. The target gene of miR-27a-3p was confirmed by the luciferase reporter assay. Results: MiR-27a-3p was downregulated in both serum and pulp tissue of pulpitis patients. MiR-27a-3p could distinguish pulpitis patients from healthy controls and might be a predictor for the development of irreversible pulpitis. A high level of TLR4 was also detected in both peripheral blood monocytes and pulp tissues from pulpitis patients and showed a negative association with the miR-27a-3p level. TLR4 was a direct target gene of miR-27a-3p. Discussion/Conclusion: MiR-27a-3p might be a promising biomarker for the diagnosis of pulpitis and predict the development of irreversible pulpitis. MiR-27a-3p might be involved in the pathogenesis of pulpitis via targeting TLR4.
Neuroimmunomodulation

中文翻译：

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miR-27a-3p 在牙髓炎中的临床价值和潜在靶点

简介：本研究调查了 miR-27a-3p 对牙髓炎患者的临床价值，及其与 TLR4 的关联。方法：招募牙髓炎患者66例，非牙髓炎患者34例；从每个参与者那里收集牙髓组织和血清样本。实时聚合酶链反应用于测量基因表达水平。通过受试者工作特征曲线评估诊断值。miR-27a-3p的靶基因通过荧光素酶报告基因测定得到证实。结果：MiR-27a-3p 在牙髓炎患者的血清和牙髓组织中均下调。MiR-27a-3p 可以区分牙髓炎患者和健康对照，并且可能是不可逆牙髓炎发展的预测因子。在牙髓炎患者的外周血单核细胞和牙髓组织中也检测到高水平的 TLR4，并且与 miR-27a-3p 水平呈负相关。TLR4是miR-27a-3p的直接靶基因。讨论/结论： MiR-27a-3p 可能是用于诊断牙髓炎和预测不可逆牙髓炎发展的有前景的生物标志物。MiR-27a-3p可能通过靶向TLR4参与牙髓炎的发病机制。
神经免疫调节