Osteosarcoma (OS) is a kind of fatal primary bone tumors in adolescents and young adults. Long noncoding RNAs (lncRNAs) are a group of noncoding RNAs which occupy a part of the latest hot topics. We aimed to investigate the roles of lncRNA LINC00665 in OS in this study. In this study, we found that LINC00665 was highly expressed in OS tissues and cell lines, and its high expression was associated with malignant feature and poor prognosis of OS. In OS cells, LINC00665 could facilitate the proliferation, migration, and invasion to play an oncogenic role. Mechanistically, LINC00665 served as a sponge for miR-708 and miR-142-5p and positively mediated the expression of their target RAP1B. Finally, we confirmed that LINC00665 exercised its biological functions by mediating RAP1B. In conclusion, LINC00665 is overexpressed in OS and facilitates the malignant processes of OS cells by increasing the RAP1B expression via sponging miR-708 and miR-142-5p.

中文翻译：

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LINC00665 通过海绵化 miR-708 和 miR-142-5p 增加 RAP1B 表达促进骨肉瘤的恶性过程

骨肉瘤（OS）是一种在青少年和年轻人中致命的原发性骨肿瘤。长链非编码RNA（lncRNAs）是一组非编码RNA，占据了最新热点的一部分。在本研究中，我们旨在调查 lncRNA LINC00665 在 OS 中的作用。在本研究中，我们发现LINC00665在OS组织和细胞系中高表达，其高表达与OS的恶性特征和预后不良有关。在 OS 细胞中，LINC00665 可以促进增殖、迁移和侵袭，从而发挥致癌作用。从机制上讲，LINC00665 充当 miR-708 和 miR-142-5p 的海绵，并正向介导其靶标 RAP1B 的表达。最后，我们证实 LINC00665 通过介导 RAP1B 发挥其生物学功能。综上所述，