Human dental pulp stem cells (hDPSCs) have significant potential of immunomodulatory for therapeutic and regenerative biomedical applications compared to other mesenchymal stem cells (MSCs). Nowadays, alteration of gene expression is an important way to improve the performance of MSCs in the clinic. MicroRNAs (miRs) and CD200 are known to modulate the immune system in MSCs. Curcumin is famous for its anti-inflammatory impacts. Phytosomal curcumin (PC) is a nanoparticle synthesized from curcumin that removes the drawbacks of curcumin. The purpose of this research was to assess the effects of PC on the expression of the CD200 and four key miRNAs in immune system. PC (30 μM) treatment of hDPSCs could ameliorate their immunoregulatory property, presented by reduced expressions of miR-21, miR-155 and miR-126, as well as enhanced expressions of miR-23 and CD200. The PC was also able to reduce PI3K\AKT1\NF-κB expressions that were target genes for these miRs and involved in inflammatory pathways. Moreover, PC was more effective than curcumin in improving the immune modulation of hDPSCs. Evidence in this study suggested that PC mediates immunoregulatory activities in hDPSC via miRs and CD200 to regulate PI3K\AKT1\NF-κB signalling pathways, which may provide a theoretical basis for PC in the treatment of many diseases.

中文翻译：

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植物体姜黄素对牙髓干细胞相关微 RNA、CD200 表达和炎症通路的免疫调节作用

与其他间充质干细胞 (MSC) 相比，人牙髓干细胞 (hDPSC) 在治疗和再生生物医学应用中具有显着的免疫调节潜力。如今，基因表达的改变是提高MSCs临床表现的重要途径。已知微 RNA (miR) 和 CD200 可调节 MSC 中的免疫系统。姜黄素以其抗炎作用而闻名。植物体姜黄素 (PC) 是一种由姜黄素合成的纳米颗粒，消除了姜黄素的缺点。本研究的目的是评估 PC 对 CD200 和免疫系统中四种关键 miRNA 表达的影响。PC (30 μM) 处理 hDPSCs 可以改善它们的免疫调节特性，表现为 miR-21、miR-155 和 miR-126 的表达降低，以及 miR-23 和 CD200 的增强表达。PC 还能够降低 PI3K\AKT1\NF-κB 表达，这些表达是这些 miR 的靶基因并参与炎症途径。此外，PC 在改善 hDPSCs 的免疫调节方面比姜黄素更有效。本研究的证据表明，PC通过miRs和CD200介导hDPSC中的免疫调节活性，调节PI3K\AKT1\NF-κB信号通路，这可能为PC治疗多种疾病提供理论依据。