Polymer Bulletin ( IF 3.1 ) Pub Date : 2021-07-08 , DOI: 10.1007/s00289-021-03822-7 M. A. Olivares-Ramírez 1 , L. López-Zamora 1 , M. G. Peña-Juárez 2 , E. J. Gutiérrez-Castañeda 3 , J. A. Gonzalez-Calderon 4
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The present work shows the implementation of the response surface methodology, fed by an experimental central composite design (CCD) to find the conditions that allow maximizing the inhibition of the microorganism Staphylococcus aureus with nanoparticles of TiO2 silanized with 3-aminopropyltriethoxysilane (APTES) and doped with Ag. In addition, poly(lactic) acid composites were prepared with these Ag/TiO2 nanoparticles with the aim to confer their antimicrobial effect. The independent variables considered were pH, AgNO3/TiO2 ratio (% w/w), and TiO2 nanoparticles concentration (g/250 mL), and as the variable of response, the length of the diameter of the halo or zone of inhibition presented by the microorganism (mm). Statistical analysis found that maximization of S. aureus inhibition occurs at intermediate levels with a value of 10 for pH and 5 g of TiO2 solids, while for the concentration of AgNO3 high levels are required, greater than 10% w/w. Likewise, the statistical significance was determined using the Student's t test and the p value; it was found that the significant effect corresponds to the concentration of AgNO3, so a second experimental CCD design equiradial with two factors was considered, estimating AgNO3 concentration and TiO2 amount, the pH at constant 10 value. The second experimental design indicated that maximization in S. aureus inhibition occurs at an AgNO3 concentration between 20 and 25% w/w with high amounts of TiO2 solids (7–8 g), with a resulting zone of inhibition between 26 and 28 mm. The quadratic model obtained, which represents the relationship between the lengths of the zone of inhibition with the variables considered, shows an adjustment of experimental data with a coefficient of determination (R2) of 0.82.
Graphic abstract
中文翻译：
响应面法在评价 Ag/TiO2 纳米颗粒对金黄色葡萄球菌的抑制作用中的应用
目前的工作显示了响应面方法的实施，由实验中心复合设计 (CCD) 提供，以找到允许最大限度地抑制微生物金黄色葡萄球菌与 3-氨基丙基三乙氧基硅烷 (APTES) 硅烷化的 TiO 2纳米颗粒的条件和掺杂 Ag。此外，用这些 Ag/TiO 2纳米颗粒制备聚乳酸复合材料，目的是赋予它们抗菌作用。考虑的自变量是 pH、AgNO 3 /TiO 2比率 (% w/w) 和 TiO 2纳米颗粒浓度 (g/250 mL)，以及作为响应变量的微生物呈现的晕圈或抑菌圈直径的长度 (mm)。统计分析发现，在 pH 值为 10 且 TiO 2固体为5 克的中间水平时，金黄色葡萄球菌抑制的最大化发生，而对于 AgNO 3的浓度则需要高水平，大于 10% w/w。同样，使用学生t检验和p值确定统计显着性；发现显着效果对应于 AgNO 3的浓度，因此考虑了具有两个因素的第二个等半径实验 CCD 设计，估计 AgNO3浓度和TiO 2用量，pH 值恒定在10 值。第二个实验设计表明，在AgNO 3浓度为 20% 到 25% w/w 和大量 TiO 2固体（7–8 g）时，金黄色葡萄球菌抑制作用最大化，产生的抑菌圈在 26 到 28 之间毫米。所获得的二次模型表示抑菌圈长度与所考虑变量之间的关系，显示了实验数据的调整，确定系数 ( R 2 ) 为 0.82。
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