The compound γ-aminobutyric acid (GABA) was widely used in various fields. To enhance the production of GABA in Escherichia coli BL21(DE3), the enzymes of the regeneration pathway of the coenzyme factor pyridoxal 5’-phosphate (PLP) were engineered. The recombinant E. coli strain was screened and identified. The initial concentrations of L-monosodium glutamate (L-MSG) had an obvious influence on the production of GABA. The highest concentration of GABA in recombinant E. coli BL21/pET28a-gadA was 5.54 g/L when the initial L-MSG concentration was 10 g/L, whereas it was 8.45 g/L in recombinant E. coli BL21/pET28a-gadA-SNO1-SNZ1 at an initial L-MSG concentration of 15 g/L. The corresponding conversion yields of GABA in these two strains were 91.0% and 92.7%, respectively. When the initial concentrations of L-MSG were more than 15 g/L, the concentrations of GABA in E. coli BL21/pET28a-gadA-SNO1-SNZ1 were significantly higher as compared to those in recombinant E. coli BL21/pET28a-gadA, and it reached a maximum of 13.20 g/L at an initial L-MSG concentration of 25 g/L, demonstrating that the introduction of the enzymes of the regeneration pathway of PLP favored to enhance the production of GABA. This study provides new insight into producing GABA effectively in E. coli BL21(DE3).

复合γ-氨基丁酸（GABA）被广泛应用于各个领域。为了提高大肠杆菌BL21(DE3)中 GABA 的产量，对辅酶因子吡哆醛 5'-磷酸 (PLP) 再生途径的酶进行了工程改造。筛选并鉴定了重组大肠杆菌菌株。L-味精（L-MSG）的初始浓度对GABA的产生有明显影响。当初始 L-MSG 浓度为 10 g/L 时，重组大肠杆菌BL21/pET28a-gadA中 GABA 的最高浓度为 5.54 g/L，而在重组大肠杆菌中为 8.45 g/LBL21/pET28a-gadA-SNO1-SNZ1 的初始 L-MSG 浓度为 15 g/L。这两个菌株对应的GABA转化率分别为91.0%和92.7%。当L-MSG初始浓度大于15 g/L时，大肠杆菌BL21/pET28a-gadA-SNO1-SNZ1中GABA的浓度显着高于重组大肠杆菌BL21/pET28a-gadA ，并且在初始 L-MSG 浓度为 25 g/L 时达到最大 13.20 g/L，表明 PLP 再生途径酶的引入有利于提高 GABA 的产生。这项研究为在大肠杆菌BL21(DE3) 中有效生产 GABA 提供了新的见解。