Investigation of antibacterial activity of polyvinyl alcohol packaging films composed of silver oxide nanoparticles, graphene oxide and tragacanth gum using Box–Behnken design,Packaging Technology and Science

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Investigation of antibacterial activity of polyvinyl alcohol packaging films composed of silver oxide nanoparticles, graphene oxide and tragacanth gum using Box–Behnken design
Packaging Technology and Science ( IF 2.8 ) Pub Date : 2021-07-07 , DOI: 10.1002/pts.2599
Shabnam Pourmoslemi ₁ , Saeid Shokouhi ₁ , Reza Mahjub ₁

Affiliation

The present study aimed to develop an antibacterial packaging system based on biodegradable polyvinyl alcohol (PVA) polymer. Box–Behnken design was used to investigate silver oxide nanoparticles, graphene oxide and tragacanth gum as film additives to obtain maximum antibacterial activity against Staphylococcus aureus and Escherichia coli. The experimental design showed the inhibitory effect of graphene oxide and tragacanth gum on the antibacterial activity of PVA films containing Ag₂O nanoparticles. The optimum film containing 5% Ag₂O nanoparticles (% of PVA weight) was prepared and characterized using scanning electron microscopy and energy-dispersive X-ray spectroscopy (SEM-EDS), Fourier transform infrared spectroscopy (FTIR) and thermogravimetric/differential thermal analyser (TG-DTA). Results of this study showed the formation of PVA film with 0.04-mm thickness, with a higher density of Ag₂O nanoparticles (25- to 75-nm size) around the upper surface. Compared with blank PVA film, the optimum film showed higher tensile strength and thermal stability. It was also more efficient in water vapour and oxygen barrier properties, along with preserving raw beef samples by inhibiting the growth of beef natural bacteria for at least 7 days, when stored in the refrigerator.

中文翻译：

使用Box-Behnken设计研究由氧化银纳米颗粒、氧化石墨烯和黄蓍胶组成的聚乙烯醇包装膜的抗菌活性

本研究旨在开发一种基于可生物降解的聚乙烯醇 (PVA) 聚合物的抗菌包装系统。Box-Behnken 设计用于研究氧化银纳米颗粒、氧化石墨烯和黄蓍胶作为薄膜添加剂，以获得对金黄色葡萄球菌和大肠杆菌的最大抗菌活性。实验设计表明氧化石墨烯和黄蓍胶对含有Ag ₂ O纳米颗粒的PVA薄膜的抗菌活性有抑制作用。含 5% Ag ₂的最佳薄膜使用扫描电子显微镜和能量色散 X 射线光谱 (SEM-EDS)、傅里叶变换红外光谱 (FTIR) 和热重/差热分析仪 (TG-DTA) 制备和表征 O 纳米粒子（PVA 重量的百分比）。这项研究的结果表明，形成了 0.04 毫米厚的 PVA 薄膜，在上表面周围具有更高密度的 Ag ₂ O 纳米颗粒（25 至 75 纳米尺寸）。与空白PVA薄膜相比，最佳薄膜表现出更高的拉伸强度和热稳定性。当储存在冰箱中时，它在水蒸气和氧气阻隔性能方面也更有效，同时通过抑制牛肉天然细菌的生长至少 7 天来保存生牛肉样品。

更新日期：2021-09-01

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