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Extraction of bioactive compounds from buriti (Mauritia flexuosa L.) fruit by eco-friendly solvents: Chemical and functional characterization
Sustainable Chemistry and Pharmacy ( IF 6 ) Pub Date : 2021-07-08 , DOI: 10.1016/j.scp.2021.100489
Pedro Ivo P. Leite 1 , Stella M.A.G. Barreto 1 , Priscilla R. Freitas 2 , Ana C.J. de Araújo 2 , Cícera L.R. Paulo 2 , Ray S. de Almeida 2 , Cristiane F. de Assis 1 , Carlos E.A. Padilha 3 , Márcio Ferrari 1 , Francisco C. de Sousa Junior 1
Affiliation  

Buriti (Mauritia flexuosa L.) is a palm tree found in several regions of Latin America. Buriti fruit is a rich source of bioactive compounds such as carotenoids and phenolic compounds. Thus, the aim of this study was to extract bioactive compounds from buriti fruit by ethanol and a supramolecular solvent system (SUPRAS) formed by octanoic acid aggregates. The extracts were evaluated for total carotenoids, β-carotene, phenolic compounds and antioxidant capacity. Additionally, SUPRAS extracts were characterized for antibacterial activity and modulating effect. The extraction of β-carotene with SUPRAS showed a yield of 5.82 ± 0.05 mg/g for the peel and 26.7 ± 0.02 mg/g for the pulp. In relation to total phenolic compounds, the yields were 32.1 ± 1.2 μg GAE/g for the peel and 24.53 ± 4.9 μg GAE/g for the pulp. The presence of gallic acid, quercetin and catechin stand out regarding the phenolics identified. The extracts showed antioxidant activity, with an emphasis on the extracts obtained by SUPRAS, which presented EC50 (concentration required to obtain a 50% antioxidant effect) for the ABTS radical sequestration of 3.00 μg/mL for the peel and 0.84 μg/mL for the pulp. When combined with norfloxacin and gentamicin antibiotics, the extracts also showed a synergistic action against multi-resistant strains of Staphylococcus aureus, Pseudomonas aeruginosa and Escherichia coli. Thus, the extraction of bioactive compounds from buriti fruit using a safe, biocompatible, biodegradable and environmentally friendly solvent such as SUPRAS represents potential for developing new pharmaceuticals, cosmetics and functional foods.



中文翻译:

用环保溶剂从 buriti ( Mauritia flexuosa L.) 果实中提取生物活性化合物:化学和功能表征

布里蒂 ( Mauritia flexuosa )L.) 是一种在拉丁美洲的几个地区发现的棕榈树。Buriti 水果富含生物活性化合物,如类胡萝卜素和酚类化合物。因此,本研究的目的是通过乙醇和由辛酸聚集体形成的超分子溶剂系统 (SUPRAS) 从 buriti 果实中提取生物活性化合物。评估提取物的总类胡萝卜素、β-胡萝卜素、酚类化合物和抗氧化能力。此外,SUPRAS 提取物具有抗菌活性和调节作用。用 SUPRAS 提取 β-胡萝卜素表明果皮的产量为 5.82 ± 0.05 mg/g,果肉的产量为 26.7 ± 0.02 mg/g。相对于总酚类化合物,果皮的产量为 32.1 ± 1.2 μg GAE/g,果肉的产量为 24.53 ± 4.9 μg GAE/g。没食子酸的存在,槲皮素和儿茶素在确定的酚类物质方面表现突出。提取物显示出抗氧化活性,重点是通过 SUPRAS 获得的提取物,它呈现了 EC50(获得 50% 抗氧化效果所需的浓度)对于 ABTS 自由基螯合,果皮为 3.00 μg/mL,果肉为 0.84 μg/mL。当与诺氟沙星和庆大霉素抗生素联合使用时,这些提取物还对金黄色葡萄球菌铜绿假单胞菌大肠杆菌的多重耐药菌株显示出协同作用。因此,使用安全、生物相容性、可生物降解和环境友好的溶剂(如 SUPRAS)从 buriti 水果中提取生物活性化合物具有开发新药物、化妆品和功能性食品的潜力。

更新日期:2021-07-08
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