Abstract

In the field of environmental toxicology, endocrine-disrupting effects have become a major concern. The present research set out to investigate the possible reproductive toxicity of acrylamide. The research was also expanded to explore the protective effects of two nutraceuticals, thymoquinone (TQ) and capsaicin, against acrylamide-induced reproductive toxicity. Six groups of sixty male albino rats were created. Group 1 was used as a control. Rats were administered a daily dose of acrylamide and acted as the model in Group 2. TQ was provided to rats once a day in Group 3. Capsaicin was administered to rats once a day in Group 4. TQ was given once daily to rats exposed to acrylamide in Group 5. Rats were given capsaicin once a day for eight weeks after being exposed to acrylamide in Group 6. Acrylamide induced oxidative stress, testicular NF-κB/p65 expression, and down-regulated the expression of occludin, all of which can contribute to its testicular toxicity, while TQ or capsaicin removes all of these toxicity signs. TQ and capsaicin have shown efficacy in alleviating all of the acrylamide's toxic insults in the current reproductive toxicity model. Both nutraceuticals upregulated the expression of occludin in testicular tissue and restored tight junction integrity, in addition to their well-known antioxidant and anti-inflammatory effects, which were confirmed in this study.

摘要

在环境毒理学领域，内分泌干扰作用已成为主要关注点。本研究着手调查丙烯酰胺可能的生殖毒性。该研究还扩展到探索两种营养保健品百里醌 (TQ) 和辣椒素对丙烯酰胺引起的生殖毒性的保护作用。产生六组六十只雄性白化大鼠。第 1 组用作对照。第 2 组大鼠每天给予丙烯酰胺剂量并作为模型。第 3 组大鼠每天给予 TQ 一次。第 4 组大鼠每天给予辣椒素一次。TQ 每天给予一次暴露于第 5 组中的丙烯酰胺。大鼠在第 6 组中暴露于丙烯酰胺后每天一次服用辣椒素，持续八周。丙烯酰胺诱导氧化应激，睾丸 NF-κB/p65 表达，并下调 occludin 的表达，所有这些都可能导致其睾丸毒性，而 TQ 或辣椒素可消除所有这些毒性迹象。TQ 和辣椒素在目前的生殖毒性模型中显示出减轻丙烯酰胺所有毒性损害的功效。除了众所周知的抗氧化和抗炎作用外，这两种营养保健品都上调了睾丸组织中 occludin 的表达并恢复了紧密连接的完整性，这在本研究中得到了证实。当前生殖毒性模型中的毒性侮辱。除了众所周知的抗氧化和抗炎作用外，这两种营养保健品都上调了睾丸组织中 occludin 的表达并恢复了紧密连接的完整性，这在本研究中得到了证实。当前生殖毒性模型中的毒性侮辱。除了众所周知的抗氧化和抗炎作用外，这两种营养保健品都上调了睾丸组织中 occludin 的表达并恢复了紧密连接的完整性，这在本研究中得到了证实。