A novel probe T-1 for the response of NTR based on fused four-ring quinoxaline skeleton was investigated. Under the action of NTR, the probe produced green fluorescence response at 495 nm. The spectroscopic properties, response mechanism, fluorescence imaging, and other aspects of probe T-1 were investigated. In the fluorescence titration experiments, the probe showed excellent selectivity, lower detection limit (41 ng/mL) and better fluorescence quantum yield (Y_u = 0.14) for NTR. The response mechanism of the probe was explained by the comparison of F-1 ultraviolet spectra obtained in two different ways and the verification of HRMS spectra. Additionally, the application of fluorescence imaging technology made it possible to detect endogenous NTR, and broadened the application prospect of fluorescent probe.

研究了一种基于稠合四环喹喔啉骨架的 NTR 响应的新型探针T - 1。在NTR的作用下，探针在495nm处产生绿色荧光响应。对探针T - 1的光谱特性、响应机制、荧光成像等方面进行了研究。在荧光滴定实验中，该探针 对NTR表现出优异的选择性、较低的检测限（41 ng/mL）和更好的荧光量子产率（Y _u = 0.14）。通过F - 1的对比说明探针的响应机制两种不同方式获得的紫外光谱和 HRMS 光谱的验证。此外，荧光成像技术的应用使得检测内源性NTR成为可能，拓宽了荧光探针的应用前景。