To investigate the variability of n-HA/PA66 composite scaffolds on differentiation and biocompatibility, we evaluated three kinds of n-HA/PA66 composite scaffolds according to the pore size and porosity. Osteogenically induced BMSCs were seeded in the three types materials and cultured in vitro, and the variability on cell adhesion, growth, proliferation, osteogenic differentiation were analyzed using scanning electronic microscopy (SEM), immunohistochemical staining, and quantitative real-time PCR analysis. Three groups’ matrices/BMSCs composites were implanted into the cranial defect of S-D rats. The differentiations of osteogenesis were then evaluated by histological and qRT-PCR analysis The vitro and vivo results showed that the group B n-HA/PA66 scaffold was more suitable for osteogenically induced BMSCs proliferation, differentiation in vitro and bone conduction in vivo than group A and C, indicating that the porous n-HA/PA66 matrices with mean pore size of 375±132.2 μm and porosity 77 ± 2.9 % has better cell biocompatibility and bone conduction.

中文翻译：

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基于n-HA/PA66复合支架不同孔径和孔隙率的生物相容性和骨传导的变异性

为了研究 n-HA/PA66 复合支架在分化和生物相容性方面的变异性，我们根据孔径和孔隙率评估了三种 n-HA/PA66 复合支架。将成骨诱导的骨髓间充质干细胞接种在三种材料中并在体外培养，并使用扫描电子显微镜（SEM）、免疫组织化学染色和定量实时 PCR 分析分析细胞粘附、生长、增殖、成骨分化的变异性。三组基质/骨髓间充质干细胞复合物植入SD大鼠颅骨缺损处。然后通过组织学和qRT-PCR分析评估成骨分化。体外和体内结果表明，B组n-HA/PA66支架更适合成骨诱导的BMSCs增殖，