This study compares salinity tolerance between red and black shell Pinctada fucata salinity stress of 20 and 50‰, while 35‰ was used as a control. The hemolymph osmotic pressure, inorganic ion concentration, the activities of Na+-K+ -ATPase, respiratory metabolism related enzymes and liver tissue antioxidant related enzymes were measured at 12 h and 24 h after salinity stress. The osmotic pressure and inorganic ion concentration of hemolymph of two strains P. fucata increased significantly with salinity. The activity of Na+-K+ -ATPase of red P. fucata only decreased under low salinity at 24 h, and was significantly higher than that the control under low salinity at 12 h and high salinity at 12 h and 24 h. The succinate dehydrogenase (SDH) activities of the P. fucata treatment groups were significantly higher than those the control at 12 h. Lactate dehydrogenase (LDH) activity increased significantly with salinity at 12 h. and the of black P. fucata LDH activity was significantly higher than the control at 24 h, while the LDH activity of red P. fucata was significantly lower than that the control in 50‰ salinity. The superoxide dismutase (SOD) activity of black P. fucata was significantly lower than that the control, while that of red P. fucata was significantly higher than that the control within 24 h. At 12 h, the catalase (CAT) activity of red P. fucata increased significantly with salinity, but decreased significantly with salinity at 24 h. The CAT activity of black P. fucata was highest at 24 h under low salinity. Glutathione peroxidase (GSH-Px) and alkaline phosphatase (AKP) activities of red P. fucata were significantly higher than those the control under low or high salinity. At high salinity for 24 h, the GSH-Px activity was lowest in black P. fucata, AKP activity was highest. The present study indicates that the physical responses of P. fucata to the salinity stress vary with shell colors. The red P. fucata can adapt to the change of environmental salinity, and such adaptability can reduce the osmotic damage caused by salinity change.

中文翻译：

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两株紫珠母贝低、高耐盐性的比较研究

本研究比较了20‰和50‰的红壳和黑壳Pinctada fucata盐度胁迫的耐盐性，而以35‰作为对照。盐胁迫后12 h和24 h测定血淋巴渗透压、无机离子浓度、Na+-K+-ATPase活性、呼吸代谢相关酶和肝组织抗氧化相关酶。2株P. fucata血淋巴渗透压和无机离子浓度随盐度升高而显着升高。红色P. fucata Na+-K+ -ATPase 活性仅在低盐度下24 h 下降，且在12 h 低盐度和高盐度下12 h 和24 h 显着高于对照。P. fucata 处理组的琥珀酸脱氢酶 (SDH) 活性在 12 h 时显着高于对照组。乳酸脱氢酶 (LDH) 活性在 12 小时随盐度显着增加。50‰盐度下，黑色P. fucata LDH活性显着高于对照，而红色P. fucata LDH活性显着低于对照。在24 h内，黑色P. fucata的超氧化物歧化酶（SOD）活性显着低于对照，而红色P. fucata的超氧化物歧化酶（SOD）活性显着高于对照。在 12 h 时，红色 P. fucata 的过氧化氢酶 (CAT) 活性随盐度升高而显着增加，但在 24 h 时随盐度升高而显着降低。在低盐度下，黑色 P. fucata 的 CAT 活性在 24 h 时最高。在低盐度或高盐度条件下，红色 P. fucata 的谷胱甘肽过氧化物酶 (GSH-Px) 和碱性磷酸酶 (AKP) 活性均显着高于对照。24 h 高盐度下，黑色 P. fucata 的 GSH-Px 活性最低，AKP 活性最高。目前的研究表明，P. fucata 对盐度胁迫的物理反应因壳颜色而异。红海参能适应环境盐度的变化，这种适应性可以减少盐度变化引起的渗透破坏。