Objectives Determination of LH in urine has proved to be a reliable method for evaluation of pubertal development. The human LH assay based on time-resolved immunofluorometric (IFMA) technology (AutoDELFIA, PerkinElmer, Wallac) has been found to be suitable for this purpose thanks to its high sensitivity but other assays have not been evaluated. We have analyzed our data obtained by another potentially sensitive detection technique, enhanced luminometric assay (LIA) with the objective of finding a viable alternative to IFMA since these may not be available in the future. Methods LIA was used to measure LH and FSH in serum and urine samples from 100 healthy subjects of each Tanner stage and both genders, whose pubertal development has been determined. Results Urinary gonodotropin concentrations measured by LIA correlated well with Tanner stage [(r=0.93 for girls, r=0.81 for boys; p<0.01 for LH) and (r=0.81 for girls, r=0.73 for boys; p<0.01 for FSH)]. LIA determinations revealed the increase in U-LH concentrations during the transition from Tanner stage 1–2 in both girls and boys (p<0.001), whereas U-FSH and S-LH were able to detect the increase from Tanner stage 1–2 only in boys or girls, respectively (both p<0.001). Conclusions Measurement of urinary gonadotropin concentrations by LIA may be useful for the evaluation of overall pubertal development and also in the detection of transition from prepuberty to puberty.

中文翻译：

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通过增强型发光测定法 (LIA) 测量尿液促性腺激素以评估青春期发育

目的 尿中 LH 的测定已被证明是评估青春期发育的可靠方法。已发现基于时间分辨免疫荧光 (IFMA) 技术（AutoDELFIA、PerkinElmer、Wallac）的人类 LH 测定法因其高灵敏度而适用于此目的，但尚未评估其他测定法。我们分析了通过另一种潜在敏感检测技术（增强型发光测定法 (LIA)）获得的数据，目的是找到一种可行的 IFMA 替代方案，因为这些技术将来可能无法使用。方法 LIA 用于测量来自 100 名各 Tanner 阶段和性别的健康受试者的血清和尿液样本中的 LH 和 FSH，其青春期发育已确定。结果 LIA 测量的尿促性腺激素浓度与 Tanner 分期密切相关 [（女孩 r=0.93，男孩 r=0.81；LH p<0.01）和（女孩 r=0.81，男孩 r=0.73；p<0.01 FSH)]。LIA 测定揭示了女孩和男孩从 Tanner 1-2 阶段过渡期间 U-LH 浓度的增加（p<0.001），而 U-FSH 和 S-LH 能够检测到 Tanner 1-2 阶段的增加分别仅在男孩或女孩中（均 p<0.001）。结论 通过 LIA 测量尿促性腺激素浓度可能有助于评估整体青春期发育以及检测从青春期前到青春期的过渡。LIA 测定揭示了女孩和男孩从 Tanner 1-2 阶段过渡期间 U-LH 浓度的增加（p<0.001），而 U-FSH 和 S-LH 能够检测到 Tanner 1-2 阶段的增加分别仅在男孩或女孩中（均 p<0.001）。结论 通过 LIA 测量尿促性腺激素浓度可能有助于评估整体青春期发育以及检测从青春期前到青春期的过渡。LIA 测定揭示了女孩和男孩从 Tanner 1-2 阶段过渡期间 U-LH 浓度的增加（p<0.001），而 U-FSH 和 S-LH 能够检测到 Tanner 1-2 阶段的增加分别仅在男孩或女孩中（均 p<0.001）。结论 通过 LIA 测量尿促性腺激素浓度可能有助于评估整体青春期发育以及检测从青春期前到青春期的过渡。