Effects of feeding dietary defatted black soldier fly (Hermetia illucens) larvae meal (HI) on intestine microbiota, and on histomorphology, oxidative enzyme activities in liver and intestine of pikeperch (Sander lucioperca) were investigated. Four isoproteic (45% crude protein) and isolipidic (18% ether extract) diets were formulated to include 0% (CO), 9% (HI9), 18% (HI18) and 36% (HI36) of HI as replacement for fishmeal at 0, 25, 50 and 100%, respectively, and were fed to triplicate groups of juvenile pikeperch (initial body weight, 68.7 ± 7.1 g) for 84 days. No adverse effects were detected on the intestine of pikeperch fed diet groups, in terms of histomorphology (P > 0.05), while fish fed free or low levels of HI (≤ 9% in diet) showed significant liver degeneration (P 0.05). Feeding HI-containing diets positively modulated the richness and diversity of intestinal microbiota, especially for HI18 group (P < 0.05). Inclusion HI up to 18% (50% fishmeal replacement) in pikeperch diets increased abundance of Clostridium, Oceanobacillus, Bacteroides and Faecalibacterium genera, whereas the predominant bacterium, Cetobacterium was found in control and HI36 groups. This study reveals the potential of HI as an immune and health booster for juvenile pikeperch.

中文翻译：

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Pikeperch (Sander lucioperca) 如何响应膳食昆虫膳食 (Hermetia illucens)？肠道微生物群、组织形态学和抗氧化生物标志物的研究

研究了饲喂脱脂黑水虻 (Hermetia illucens) 幼虫粉 (HI) 对肠道微生物群的影响，以及对梭鲈 (Sander lucioperca) 肝脏和肠道组织形态学、氧化酶活性的影响。四种异蛋白（45% 粗蛋白）和异脂（18% 乙醚提取物）日粮被配制为包括 0% (CO)、9% (HI9)、18% (HI18) 和 36% (HI36) 的 HI 作为鱼粉的替代品分别以 0%、25%、50% 和 100% 的浓度喂给三份平行的鲈鱼幼鱼组（初始体重，68.7 ± 7.1 克），持续 84 天。在组织形态学方面，未检测到梭鱼喂养组的肠道有不良影响（P > 0.05），而无或低水平 HI（饮食中≤ 9%）喂养的鱼表现出显着的肝脏退化（P = 0.05）。喂食含 HI 的饮食对肠道微生物群的丰富度和多样性有积极调节作用，尤其是 HI18 组（P < 0.05）。在梭鲈饮食中加入高达 18%（50% 鱼粉替代品）的 HI 增加了梭菌、海洋杆菌、拟杆菌和粪杆菌属的丰度，而在对照组和 HI36 组中发现了优势细菌 Cetobacterium。这项研究揭示了 HI 作为梭鲈幼鱼免疫和健康促进剂的潜力。