Endometritis is among the most common bovine uterine diseases; as a cause of infertility, it affects the progress of the cattle industry. In this study, we used a novel technique based on ultra-performance liquid chromatography/quadrupole time-of-flight mass spectrometry for comparative metabolomics of uterine secretions in healthy cows and cows with endometritis, classified based on clinical symptoms. Univariate and multivariate statistical analyses revealed significant differences between the two groups (n = 12). Compared with healthy uterine secretion samples, in the clinical endometritis samples, coumaric acid, benzoic acid, and equol were downregulated. However, l-phenylalanine, glutamine, succinic acid, linoleate, arachidonic acid, and other metabolites were upregulated, revealing variations between healthy cows and cows with endometritis (p < 0.05). This metabolomic approach may provide an in-depth understanding of endometritis pathobiology, along with a theoretical framework for the diagnosis and treatment of this bovine disease.

子宫内膜炎是最常见的牛子宫疾病之一。作为不孕症的一个原因，它影响了养牛业的进步。在本研究中，我们使用基于超高效液相色谱/四极杆飞行时间质谱的新技术对健康奶牛和子宫内膜炎奶牛的子宫分泌物进行比较代谢组学，根据临床症状进行分类。单变量和多变量统计分析显示两组之间存在显着差异（n  = 12）。与健康子宫分泌物样本相比，在临床子宫内膜炎样本中，香豆酸、苯甲酸和雌马酚被下调。然而，l-苯丙氨酸、谷氨酰胺、琥珀酸、亚油酸、花生四烯酸和其他代谢物上调，揭示了健康奶牛和患有子宫内膜炎的奶牛之间的差异（p  < 0.05）。这种代谢组学方法可以提供对子宫内膜炎病理生物学的深入理解，以及诊断和治疗这种牛病的理论框架。