The main problem for submerged fermentation of filamentous fungi is the nutrition limitation with high cell density or cell leakage by the uncontrollable hyphae, clusters, or pellets. There are several techniques such as microparticle, immobilization, pH shifting, substrate limitation etc. for controlling filamentous fungi growth on submerged fermentation. In this research, FDM (Fused Deposition Modelling) based 3D printed cubes is used for growth control agent of recombinant Aspergillus sojae for the first time. Lattice structure sizes, number of cubes and pH were chosen to be main factors of fermentation in order to study the combine effect of the factors on A. sojae fermentation. The results revealed that specific activity values are improved from 2045.96 U/mg (the highest control activity) to 3291.67 U/mg with lower pellet sizes and controllable growth. FDM based 3D printed cubes was successfully controlled the recombinant Aspergillus sojae fermentation and enhanced β-mannanase production. In addition, this research was also showed that FDM based 3D printed cubes also have the potential to be used as immobilization materials like SLS based 3D printed products in further research.

丝状真菌深层发酵的主要问题是由于无法控制的菌丝、簇或颗粒造成的高细胞密度或细胞渗漏的营养限制。有多种技术，例如微粒、固定化、pH 变化、底物限制等，可用于控制深层发酵中丝状真菌的生长。在这项研究中，基于 FDM（熔融沉积建模）的 3D 打印立方体首次用于重组酱油曲霉的生长控制剂。选择晶格结构大小、立方体数量和pH值作为发酵的主要因素，以研究这些因素对酱油曲霉的综合影响发酵。结果表明，比活性值从 2045.96 U/mg（最高对照活性）提高到 3291.67 U/mg，颗粒尺寸较小，生长可控。基于 FDM 的 3D 打印立方体成功控制了重组酱油曲霉发酵并增强了 β-甘露聚糖酶的产生。此外，这项研究还表明，基于 FDM 的 3D 打印立方体也有可能在进一步研究中用作固定材料，如基于 SLS 的 3D 打印产品。