Monitoring microplastics in the environment based on the Nile red staining protocol has proven to be a newly emerged method in several instances. However, the methodology is still having the limitations of susceptibility, indiscrimination, and complexity, etc. The objectives of this paper are to explore the effects of wavelength, temperature, H₂O₂ and NaCl addition, plastic property, and fluorescent index on the Nile red staining in microplastics analysis and propose solutions to these inadequacies. Sample co-stained with H₂O₂ (ω_final = 10%) and NaCl (ω_final = 8.8%) will lower the fluorescence intensity of biogenic materials and reduce their interferences. Based on the fluorescence color and intensity of fused fluorographs, the combined fluorescent index for twelve microplastics was significantly different, thus could be preliminarily distinguished. An elevated staining temperature is propitious to fluorescent tagging with Nile Red. Finally, an improved protocol was proposed, which made the methodology streamlined in microplastics analysis.

在一些情况下，基于尼罗河红染色协议监测环境中的微塑料已被证明是一种新出现的方法。然而，该方法仍然存在敏感性、无差别性和复杂性等局限性。本文的目的是探讨波长、温度、H ₂ O ₂和NaCl 添加量、塑性和荧光指数对材料的影响。微塑料分析中的尼罗红染色并针对这些不足提出解决方案。样品与 H ₂ O ₂ (ω _final  = 10%) 和 NaCl (ω _final = 8.8%) 将降低生物材料的荧光强度并减少它们的干扰。根据融合荧光照片的荧光颜色和强度，12种微塑料的组合荧光指数有显着差异，可以初步区分。升高的染色温度有利于用尼罗红进行荧光标记。最后，提出了一种改进的协议，使微塑料分析中的方法流线化。