The outbreaks of hepatitis-hydropericardium syndrome (HPS) caused by serotype 4 fowl adenovirus (FAdV-4) have spread from broilers to laying hens, breeders, geese and Cherry Valley duck, resulting in high economic losses to the poultry industry globally. In this study, a rapid colloidal gold test strip for detection of FAdV-4 was developed by using two monoclonal antibodies (mAbs) against the Fiber-2 of FAdV-4. Specificity analysis revealed that the test strip only reacted with FAdV-4, but not with other pathogens including different serotypes of fowl adenovirus and other avian pathogens tested. The limit of the detection (LOD) of the strip could reach as low as 0.1 μg/0.1 mL of GST-Fiber-2 protein and 1 × 10⁵ TCID₅₀/0.1 mL of FAdV-4, respectively. Moreover, the test strip could be efficiently applied in detecting tissue samples from diseased chickens with HPS. Comparison analysis further revealed that the test strip showed good compatibility with PCR assay for detection of virus isolates and clinical samples. In conclusion, our test strip provides an efficient on-site diagnostic method in a quick and convenient manner for detection of FAdV-4, especially in resource-limited areas.

由血清型 4 禽腺病毒 (FAdV-4) 引起的肝炎-心包积水综合征 (HPS) 暴发已从肉鸡蔓延到蛋鸡、种鸡、鹅和樱桃谷鸭，给全球家禽业造成了巨大的经济损失。在本研究中，通过使用两种针对 FAdV-4 的 Fiber-2 的单克隆抗体 (mAb)，开发了一种用于检测 FAdV-4 的快速胶体金试纸条。特异性分析表明，该测试条仅与 FAdV-4 发生反应，但不与其他病原体（包括不同血清型的禽腺病毒和其他受试禽类病原体）发生反应。条带的检测限 (LOD) 可低至 0.1 μg/0.1 mL GST-Fiber-2 蛋白和 1 × 10 ⁵ TCID ₅₀/0.1 mL 的 FAdV-4，分别。此外，该测试条可以有效地用于检测 HPS 患病鸡的组织样本。比较分析进一步表明，该试纸与用于检测病毒分离株和临床样本的 PCR 检测具有良好的兼容性。总之，我们的测试条以快速方便的方式提供了一种有效的现场诊断方法，用于检测 FAdV-4，尤其是在资源有限的地区。