Breast cancer (BC) is the most invasive cancer with different subtypes that its metabolism is unique compared with normal cells. Glutamine is considered critical nutrition that many cancer cells, particularly BC cells, are dependent on it for growth and proliferation. Therefore, targeting glutamine metabolism, especially enzymes that are related to this pathway, can be beneficial to design anti-cancer agents. Recent evidence has shown that microRNAs (miRNAs), with a short length and single-strand properties, play a prominent role in regulating the genes related to glutamine metabolism, which may control the development of cancer. In silico analysis confirmed that miR-513c and miR-3163 might be involved in glutamine metabolism. The expression level of these two miRNAs was evaluated in eighty BC tissues and normal adjacent tissues. Furthermore, GSE38167, GSE38867, GSE42128, GSE45666, and GSE53179 were employed from gene expression omnibus (GEO). The Limma package was utilized to identify differentially expressed miRNAs (DEMs) of mentioned datasets to evaluate miR-513c and miR-3163 expression. Further, in silico analysis was utilized to predict the potential biological processes and molecular pathways of miR-513c and miR-3163, based on their target genes. In silico studies revealed top categories of biological processes and cellular pathways that might play a critical role in metabolism reprogramming and cancer development and were target genes for miR-513c and miR-3163. The current study showed that miR-513c (p value = 0.02062 and FC = − 2.3801) and miR-3163 (p value = 0.02034 and FC = − 2.3792) were downregulated in tumor tissues compared to normal adjacent tissues. The analysis of GEO microarray datasets showed that miR-513c was downregulated in GSE38167, GSE38867, GSE42128, GSE45666 and GSE53179, whereas there was a significant downregulation of miR-3163 in only two studies, including GSE38867 and GSE42128 that they were in accordance with our experimental results. Furthermore, the subgroup analysis did not show any substantial relationship between expression levels of these two miRNAs and factors such as age, family history of cancer, and abortion history. MiR-513c and miR-3163 were downregulated in BC tissues, which might serve as tumor suppressors. They are suggested as potential therapeutic targets for patients with BC.

中文翻译：

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与乳腺癌患者的正常癌旁组织相比，miR-513c和miR-3163在肿瘤组织中的表达下调

乳腺癌（BC）是最具侵袭性的癌症，具有不同亚型，其代谢与正常细胞相比是独特的。谷氨酰胺被认为是重要的营养物质，许多癌细胞，特别是 BC 细胞，都依赖它来生长和增殖。因此，针对谷氨酰胺代谢，尤其是与该途径相关的酶，可能有利于设计抗癌药物。最近的证据表明，具有短长度和单链特性的微小RNA（miRNA）在调节与谷氨酰胺代谢相关的基因中起重要作用，这可能控制癌症的发展。计算机分析证实 miR-513c 和 miR-3163 可能参与谷氨酰胺代谢。在 80 个 BC 组织和正常邻近组织中评估了这两种 miRNA 的表达水平。此外，GSE38167、GSE38867、GSE42128、GSE45666 和 GSE53179 来自基因表达综合 (GEO)。Limma 包用于识别上述数据集的差异表达 miRNA (DEM)，以评估 miR-513c 和 miR-3163 的表达。此外，计算机分析用于预测 miR-513c 和 miR-3163 的潜在生物学过程和分子途径，基于它们的靶基因。计算机研究揭示了可能在代谢重编程和癌症发展中发挥关键作用的顶级生物过程和细胞途径，并且是 miR-513c 和 miR-3163 的靶基因。目前的研究表明，与正常邻近组织相比，miR-513c（p 值 = 0.02062 和 FC = - 2.3801）和 miR-3163（p 值 = 0.02034 和 FC = - 2.3792）在肿瘤组织中下调。GEO 微阵列数据集的分析显示 miR-513c 在 GSE38167、GSE38867、GSE42128、GSE45666 和 GSE53179 中下调，而仅在两项研究中 miR-3163 显着下调，包括 GSE38867 和 GSE42128，它们与我们的研究一致。实验结果。此外，亚组分析未显示这两种 miRNA 的表达水平与年龄、癌症家族史和流产史等因素之间存在任何实质性关系。MiR-513c 和 miR-3163 在 BC 组织中被下调，这可能作为肿瘤抑制因子。它们被建议作为 BC 患者的潜在治疗靶点。而仅在两项研究中miR-3163显着下调，包括GSE38867和GSE42128，它们与我们的实验结果一致。此外，亚组分析未显示这两种 miRNA 的表达水平与年龄、癌症家族史和流产史等因素之间存在任何实质性关系。MiR-513c 和 miR-3163 在 BC 组织中被下调，这可能作为肿瘤抑制因子。它们被建议作为 BC 患者的潜在治疗靶点。而仅在两项研究中miR-3163显着下调，包括GSE38867和GSE42128，它们与我们的实验结果一致。此外，亚组分析未显示这两种 miRNA 的表达水平与年龄、癌症家族史和流产史等因素之间存在任何实质性关系。MiR-513c 和 miR-3163 在 BC 组织中被下调，这可能作为肿瘤抑制因子。它们被建议作为 BC 患者的潜在治疗靶点。MiR-513c 和 miR-3163 在 BC 组织中被下调，这可能作为肿瘤抑制因子。它们被建议作为 BC 患者的潜在治疗靶点。MiR-513c 和 miR-3163 在 BC 组织中被下调，这可能作为肿瘤抑制因子。它们被建议作为 BC 患者的潜在治疗靶点。