Comparative immunogenicity of outer membrane protein K and whole-cell antigens of Vibrio parahaemolyticus for diagnosis,Letters in Applied Microbiology

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Comparative immunogenicity of outer membrane protein K and whole-cell antigens of Vibrio parahaemolyticus for diagnosis
Letters in Applied Microbiology ( IF 2.0 ) Pub Date : 2021-07-07 , DOI: 10.1111/lam.13536
W Wang _{1,

2,

3} , S Guo _{1,

2} , Y Gao _{1,

2} , X Liang _{1,

2} , L Liu _{1,

2} , S Pan _{1,

2,

3}

Affiliation

The immunogenicity of soluble outer membrane protein K (OmpK)- small ubiquitin-like modifier, OmpK inclusion bodies, formalin, and heat-killed Vibrio parahaemolyticus cells were prepared and studied in a mouse model. The results of whole-cell ELISA and Western blot (WB) revealed that the serum against soluble OmpK and OmpK inclusion bodies reacted only with homologous V. parahaemolyticus. Furthermore, recombinant OmpK proteins were not recognized by the serum against whole-cell V. parahaemolyticus antigens. Unexpectedly, the serum against formalin and heat-killed V. parahaemolyticus reacted broadly with homologous (an immunization strain) and heterologous (non-immunization strains) V. parahaemolyticus and Vibrio species. The WB results revealed that the serum against the two V. parahaemolyticus whole-cell antigens primarily reacted with proteins that were approximately 100, 70, 36, 28, and 22 kDa in the cell lysates from different Vibrio strains, rather than the recombinant OmpK. The 70 and 28 kDa proteins exhibited specificity to Vibrio species, while the 22 kDa protein was more specific to V. parahaemolyticus. This study showed the limitation of recombinant OmpK to prepare diagnostic antibodies and revealed several specific Omps of Vibrio sp. and V. parahaemolyticus that were promising in diagnosis and vaccine development.

中文翻译：

副溶血性弧菌外膜蛋白K和全细胞抗原的比较免疫原性诊断

在小鼠模型中制备可溶性外膜蛋白 K (OmpK)-小泛素样修饰剂、OmpK 包涵体、福尔马林和热灭活的副溶血性弧菌细胞的免疫原性。全细胞ELISA和蛋白质印迹(WB)结果显示抗可溶性OmpK和OmpK包涵体的血清仅与同源副溶血性弧菌反应。此外，针对全细胞副溶血性弧菌抗原的血清不能识别重组 OmpK 蛋白。出乎意料的是，针对福尔马林和热灭活的副溶血性弧菌的血清与同源（免疫菌株）和异源（非免疫菌株）副溶血性弧菌和弧菌种。WB 结果显示，针对两种副溶血性弧菌全细胞抗原的血清主要与来自不同弧菌菌株的细胞裂解物中大约 100、70、36、28和 22 kDa 的蛋白质反应，而不是与重组 OmpK 反应。70 kDa 和 28 kDa 蛋白质对弧菌物种表现出特异性，而 22 kDa 蛋白质对副溶血弧菌更具特异性。该研究显示了重组 OmpK 在制备诊断抗体方面的局限性，并揭示了弧菌属的几种特定 Omp 。和V. parahaemolyticus在诊断和疫苗开发方面很有前景。

更新日期：2021-07-07

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