CCCH is a subfamily of zinc finger proteins involved in plant growth, development, and stresses response. The function of CCCH in regulating leaf senescence, especially its roles in abscisic acid (ABA)-mediated leaf senescence is largely unknown. The objective of this study was to determine functions and mechanisms of CCCH gene in regulating leaf senescence in switchgrass (Panicum virgatum). A CCCH gene, PvCCCH69 (PvC3H69), was cloned from switchgrass. Overexpressing PvC3H69 in rice suppressed both natural senescence with leaf aging and dark-induced leaf senescence. Endogenous ABA content, ABA biosynthesis genes (NCED3, NCED5, and AAO3), and ABA signaling-related genes (SnRKs, ABI5, and ABF2/3/4) exhibited significantly lower levels in senescencing leaves of PvC3H69-OE plants than those in WT plants. PvC3H69-suppression of leaf senescence was associated with transcriptional upregulation of genes mainly involved in the light-dependent process of photosynthesis, including light-harvesting complex proteins, PSI proteins, and PSII proteins and downregulation of ABA biosynthesis and signaling genes and senescence-associated genes. PvC3H69 could act as a repressor for leaf senescence via upregulating photosynthetic proteins and repressing ABA synthesis and ABA signaling pathways.

CCCH 是参与植物生长、发育和胁迫响应的锌指蛋白亚家族。CCCH 在调节叶片衰老中的功能，特别是其在脱落酸 (ABA) 介导的叶片衰老中的作用在很大程度上是未知的。本研究的目的是确定CCCH基因在调控柳枝稷( Panicum virgatum )叶片衰老中的功能和机制。甲CCCH基因，PvCCCH69（PV C3H69），由柳枝稷克隆。在水稻中过表达 Pv C3H69抑制了叶片老化的自然衰老和黑暗诱导的叶片衰老。内源 ABA 含量、ABA 生物合成基因（NCED3、NCED5和AAO3）) 和 ABA 信号相关基因 ( SnRKs、ABI5和ABF2/3/4 ) 在PvC3H69- OE 植物的衰老叶片中表现出显着低于WT 植物中的水平。Pv C3H69 - 叶片衰老的抑制与主要参与光合作用光依赖过程的基因的转录上调有关，包括光捕获复合蛋白、PSI 蛋白和 PSII 蛋白以及 ABA 生物合成和信号基因的下调以及衰老相关基因基因。PvC3H69可以通过上调光合蛋白和抑制 ABA 合成和 ABA 信号通路来抑制叶片衰老。