CCCH is a subfamily of zinc finger proteins involved in plant growth, development, and stresses response. The function of CCCH in regulating leaf senescence, especially its roles in abscisic acid (ABA)-mediated leaf senescence is largely unknown. The objective of this study was to determine functions and mechanisms of CCCH gene in regulating leaf senescence in switchgrass (Panicum virgatum). A CCCH gene, PvCCCH69 (PvC3H69), was cloned from switchgrass. Overexpressing PvC3H69 in rice suppressed both natural senescence with leaf aging and dark-induced leaf senescence. Endogenous ABA content, ABA biosynthesis genes (NCED3, NCED5, and AAO3), and ABA signaling-related genes (SnRKs, ABI5, and ABF2/3/4) exhibited significantly lower levels in senescencing leaves of PvC3H69-OE plants than those in WT plants. PvC3H69-suppression of leaf senescence was associated with transcriptional upregulation of genes mainly involved in the light-dependent process of photosynthesis, including light-harvesting complex proteins, PSI proteins, and PSII proteins and downregulation of ABA biosynthesis and signaling genes and senescence-associated genes. PvC3H69 could act as a repressor for leaf senescence via upregulating photosynthetic proteins and repressing ABA synthesis and ABA signaling pathways.

CCCH 是参与植物生长、发育和胁迫响应的锌指蛋白亚家族。 CCCH 在调节叶片衰老中的功能，特别是其在脱落酸 (ABA) 介导的叶片衰老中的作用尚不清楚。本研究的目的是确定CCCH基因在调节柳枝稷（ Panicum virgatum ）叶片衰老中的功能和机制。 CCCH基因PvCCCH69 (Pv C3H69)是从柳枝稷中克隆的。在水稻中过表达 Pv C3H69可以抑制叶片自然衰老和黑暗诱导的叶片衰老。 PvC3H69 -OE植物衰老叶片中的内源ABA含量、ABA生物合成基因（ NCED3、NCED5和AAO3 ）和ABA信号相关基因（ SnRKs 、 ABI5和ABF2/3/4 ）的水平显着低于WT植物植物。 Pv C3H69 -叶片衰老的抑制与主要参与光合作用光依赖性过程的基因转录上调有关，包括光捕获复合蛋白、PSI 蛋白和 PSII 蛋白，以及 ABA 生物合成和信号基因的下调以及衰老相关基因的转录上调。基因。 PvC3H69可以通过上调光合蛋白并抑制 ABA 合成和 ABA 信号通路来抑制叶片衰老。