The literature indicates that LINC00174 inhibits the growth of colorectal cancer (CRC) cells, but its research needs to be enriched. We tried to explore the function and mechanism of LINC00174 in CRC cell proliferation and migration. Bioinformatics analysis predicted the binding relationship and expressions of lncRNA, miRNA and mRNA. Clinical study analyzes the relationship between LINC00174 and clinical data characteristics of CRC patients. The expressions of LINC00174, miR-3127-5p and E2F7 were verified by RT-qPCR, and the combination of the two was verified by dual luciferase analysis and RNA immunoprecipitation as needed. Western blot was used to detect the expression of EMT-related protein and E2F7 protein. Functional experiments were used to evaluate the function of the target gene on CRC cells. LINC00174 was up-regulated in CRC clinical samples and cells and was related to the clinical characteristics of CRC patients. High-expression of LINC00174, contrary to the effect of siLINC00174, promoted cell viability, proliferation, migration and invasion, up-regulated the expressions of N-Cadherin, Vimentin, E2F7, and inhibited the expression of E-Cadherin. MiR-3127-5p was one of the targeted miRNAs of LINC00174 and was down-regulated in CRC samples. In addition, miR-3127-5p mimic partially reversed the malignant phenotype of CRC cells induced by LINC00174. Besides, E2F7 was a target gene of miR-3127-5p, and LINC00174 repressed miR-3127-5p to regulate E2F7. Our research reveals that LINC00174 affected the biological characteristics of CRC cells through regulated miR-3127-5p/ E2F7 axis.

中文翻译：

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LINC00174 通过 MiR-3127-5p/ E2F7 轴促进结直肠癌细胞的增殖和迁移。

文献表明LINC00174抑制结直肠癌（CRC）细胞的生长，但其研究有待丰富。我们试图探索LINC00174在CRC细胞增殖和迁移中的作用和机制。生物信息学分析预测了 lncRNA、miRNA 和 mRNA 的结合关系和表达。临床研究分析了LINC00174与CRC患者临床数据特征之间的关系。通过RT-qPCR验证LINC00174、miR-3127-5p和E2F7的表达，根据需要通过双荧光素酶分析和RNA免疫沉淀验证两者的结合。Western blot检测EMT相关蛋白和E2F7蛋白的表达。功能实验用于评估靶基因对CRC细胞的功能。LINC00174 在 CRC 临床样本和细胞中上调，并且与 CRC 患者的临床特征相关。LINC00174的高表达与siLINC00174的作用相反，促进细胞活力、增殖、迁移和侵袭，上调N-Cadherin、Vimentin、E2F7的表达，抑制E-Cadherin的表达。MiR-3127-5p 是 LINC00174 的靶向 miRNA 之一，在 CRC 样本中被下调。此外，miR-3127-5p mimic 部分逆转了 LINC00174 诱导的 CRC 细胞的恶性表型。此外，E2F7是miR-3127-5p的靶基因，LINC00174通过抑制miR-3127-5p来调控E2F7。我们的研究表明，LINC00174 通过调节 miR-3127-5p/E2F7 轴影响 CRC 细胞的生物学特性。