Camellia nitidissima Chi leaf as pancreatic lipase inhibitors: Inhibition potentials and mechanism,Journal of Food Biochemistry

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Camellia nitidissima Chi leaf as pancreatic lipase inhibitors: Inhibition potentials and mechanism
Journal of Food Biochemistry ( IF 3.5 ) Pub Date : 2021-07-06 , DOI: 10.1111/jfbc.13837
Jiahui Chen ₁ , Xuehui Wu ₁ , Yue Zhou ₁ , Junhua He ₁

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In this study, Camellia nitidissima Chi leaf extract was investigated for its compounds and pancreatic lipase inhibitory potentials. The interaction was determined using ultraviolet (UV) spectroscopy, circular dichroism (CD), fluorescence spectroscopy (FS), and molecular docking to understand the inhibiton, kinetic, and conformation of extraction–pancreatic lipase complex. C. nitidissima Chi leaf extraction inhibited the pancreatic lipase activity in a dose-dependent manner at the concentration of 1–12 mg/ml. The Lineweaver–Burk plots indicated that the inhibition on pancreatic lipase by extraction was noncompetitive. In addition, the decrease in α-helix contents, increase in β-sheet and β-turn, and decrease in fluorescence intensity after extraction treatment indicated that the conformation of pancreatic lipase was changed. This work revealed that C. nitidissima Chi leaf extraction played a significant role in inhibiting pancreatic lipase activity and brought out a solution of delay fat accumulation.

中文翻译：

山茶花作为胰脂肪酶抑制剂：抑制潜力和机制

在这项研究中，研究了山茶树叶提取物的化合物和胰脂肪酶抑制潜力。使用紫外 (UV) 光谱、圆二色性 (CD)、荧光光谱 (FS) 和分子对接来确定相互作用，以了解提取-胰腺脂肪酶复合物的抑制作用、动力学和构象。C. nitidissima Chi 叶提取物在 1-12 mg/ml 的浓度下以剂量依赖性方式抑制胰脂肪酶活性。Lineweaver-Burk 图表明提取对胰脂肪酶的抑制是非竞争性的。此外，α的减少β-折叠和β-转角的含量增加，提取处理后荧光强度降低，表明胰脂肪酶的构象发生了改变。这项工作揭示了C. nitidissima Chi叶提取物在抑制胰脂肪酶活性方面发挥了显着作用，并提出了延迟脂肪积累的解决方案。

更新日期：2021-09-06

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