The economic loss and the failure of the chemical control measures to impede downy mildew infection on the grapevine (Vitis vinifera L.) have led to the exploration of biocontrol agents that can effectively manage the invading disease by invoking defense responses of the host plant. Hence, the present study was aimed to explore the efficacy of the talc-based formulation of biocontrol agent Trichoderma harzianum to induce protection against oomycete pathogen, Plasmopara viticola (Berk et Curt.) Berl. et de Toni by triggering defense mechanisms in grapevine. The field study demonstrated that priming with T. harzianum led to the elicitation of defense responses in pathogen inoculated grapevines, with maximum disease protection up to 82.9% on the 4th-day. In addition, the microscopic observation of histochemicals revealed an increased accumulation of lignin, callose and hydrogen peroxide initiated at early 12 h and reached the highest at 24 h post-inoculation. Furthermore, the activities of the defense enzymes, namely, phenylalanine ammonia-lyase, peroxidase and 1,3-glucanase, were upregulated in the tripartite system between the course of tested time intervals. Overall, the findings from the present study highlighted the induced protection incited by T. harzianum in response to P. viticola infestation and witness the significant role of cellular and biochemical enzymes priming for defense in conferring protection against downy mildew disease in grapevine.

经济损失和化学防治措施未能阻止葡萄藤 ( Vitis vinifera L.)感染霜霉病，促使人们探索生物防治剂，通过调用寄主植物的防御反应来有效控制入侵疾病。因此，本研究旨在探索基于滑石粉的生物防治剂哈茨木霉制剂诱导针对卵菌病原体Plasmopara viticola (Berk et Curt.) Berl 的保护作用。et de Toni 通过触发小道消息中的防御机制。现场研究表明，用哈茨木霉引发导致在接种病原体的葡萄藤中引发防御反应，第 4 天的最大疾病保护率高达 82.9%。此外，组织化学物质的显微观察显示，木质素、胼胝质和过氧化氢的积累在早期 12 小时开始增加，并在接种后 24 小时达到最高。此外，防御酶的活性，即苯丙氨酸解氨酶、过氧化物酶和 1,3-葡聚糖酶，在测试时间间隔过程之间的三方系统中被上调。总体而言，本研究的结果强调了哈茨木霉对葡萄球菌引起的诱导保护 侵染并见证细胞和生化酶启动防御的重要作用，在葡萄藤中赋予抵抗霜霉病的保护作用。