Analysis of the Effect of Vitamin C at IC50 on RAW264.7 and K562 Cells Based on 1H NMR Metabonomics,ACS Food Science & Technology

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Analysis of the Effect of Vitamin C at IC50 on RAW264.7 and K562 Cells Based on 1H NMR Metabonomics
ACS Food Science & Technology ( IF 2.6 ) Pub Date : 2021-07-07 , DOI: 10.1021/acsfoodscitech.1c00125
Hui Li ₁ , Yang Lu ₁ , Yue Geng ₁

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Daily use of vitamin C as a health care supplement is widespread, but toxic effects of excessive doses or long-term usage of vitamin C are not well studied. Here, we first determined the IC₅₀ of vitamin C using RAW264 cells (920 mM) and K562 cells (800 mM) using an in vitro cytotoxic assay. Next, cell extracts were obtained 24 h after vitamin C treatment at IC₅₀ and analyzed for differential metabolites by nuclear magnetic resonance (¹H NMR) metabonomics using multivariate statistical analysis software. The results showed that there was a significant difference (p < 0.01) in cell growth between the vitamin C group and the control group in both RAW264.7 cells and K562 cells. We found that vitamin C treatment affected the galactose metabolism, methionine metabolism, inositol metabolism, glyceride metabolism, and glycine, serine, and threonine metabolism in both cell lines. This study provides a theoretical basis for exploring the toxic effects of high doses of vitamin C.

中文翻译：

基于¹ H NMR 代谢组学分析维生素 C 在 IC ₅₀对 RAW264.7 和 K562 细胞的影响

日常使用维生素 C 作为保健补充剂很普遍，但过量服用或长期使用维生素 C 的毒性作用尚未得到充分研究。在这里，我们首先使用 RAW264 细胞 (920 mM) 和 K562 细胞 (800 mM) 通过体外细胞毒性试验测定了维生素 C的 IC ₅₀。接下来，在 IC _{50 下}维生素 C 处理 24 小时后获得细胞提取物，并使用多变量统计分析软件通过核磁共振 ( ¹ H NMR) 代谢组学分析差异代谢物。结果表明有显着差异（p< 0.01) 在 RAW264.7 细胞和 K562 细胞中维生素 C 组和对照组之间的细胞生长。我们发现维生素 C 处理影响了两种细胞系中的半乳糖代谢、蛋氨酸代谢、肌醇代谢、甘油酯代谢以及甘氨酸、丝氨酸和苏氨酸代谢。本研究为探索高剂量维生素C的毒性作用提供了理论依据。

更新日期：2021-07-16

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