Jute is an essential fibrous crop worldwide. However, salt- and drought-induced stresses affect its production. The complex inheritance for such traits makes Quantitative Trait Loci (QTL) detection cumbersome when using the approach to understand and improve the plant’s tolerance to these stresses. There is a need to identify the underlying mechanistic events under these stress conditions at the seedling stage. Therefore, this study uses genetic and transcriptomic approaches to identify the markers, QTL and candidate genes related to salt and drought tolerance in jute. We also analyzed some phenotypes, including dry root weight (DRW), dry shoot weight (DSW), root fresh weight (RFW), root length (RL), relative water content (RWC), shoot fresh weight (SFW) and Shoot length (SL). One hundred (100) recombinant inbred lines (RILs) of the white Jute and the parents were evaluated under control, salt and drought conditions. Analysis of variance indicates significant differences (p < 0.01) among the RILs, and the estimates of broad-sense heritability of the RILs ranges from 50.0 to 98.5%. Two QTL for RL and SL under drought (qRLD-1–1 and qSLD-1–1, respectively) were found in the same position and associated with the flanking markers Marker5166 and Marker7753. Moreover, qRWCS-1–1 for the relative water content under salt treatment had the highest Phenotypic Variation Explained (PVE, 24.63%) with the flanking markers Marker19007 and Marker28120. Our results suggest that these genes regulate the two stresses at the seedling stage in Corchorus capsularis and function in different pathways. However, further study is needed to validate this. Higher additive effects were observed in sodium chloride (NaCl)-induced stress which suggest that salt stress selection would be more effective and useful at the seedling stage. The relative expression analysis of the candidate genes and the phenotypes of the samples reveal that CCACVL1_12635 and CCACVL1_15402, and CCACVL1_23216 genes are the salt and drought tolerance candidate genes, respectively, and function in the cystatin and potassium ion transporter 1 (KT1) pathways.

黄麻是世界范围内必不可少的纤维作物。然而，盐和干旱引起的胁迫会影响其生产。当使用该方法了解和提高植物对这些胁迫的耐受性时，此类性状的复杂遗传使得定量性状基因座 (QTL) 检测变得繁琐。需要在幼苗阶段确定这些胁迫条件下的潜在机制事件。因此，本研究利用遗传和转录组学方法来鉴定黄麻耐盐耐旱性相关的标记、QTL和候选基因。我们还分析了一些表型，包括干根重（DRW）、干枝重（DSW）、根鲜重（RFW）、根长（RL）、相对含水量（RWC）、枝条鲜重（SFW）和枝长(SL)。在对照、盐和干旱条件下评估了一百 (100) 个重组自交系 (RIL) 的白黄麻和亲本。方差分析表明差异显着（p  < 0.01) 在 RILs 中，RILs 的广义遗传力的估计范围从 50.0% 到 98.5%。干旱条件下 RL 和 SL 的两个 QTL（分别为qRLD-1-1和qSLD-1-1）被发现在相同位置并与侧翼标记 Marker5166 和 Marker7753 相关。此外，盐处理下相对水含量的qRWCS-1-1具有最高的表型变异解释（PVE，24.63%），侧翼标记为 Marker19007 和 Marker28120。我们的研究结果表明，这些基因在Corchorus capsularis幼苗期调节两种胁迫并在不同途径中发挥作用。但是，需要进一步研究来验证这一点。在氯化钠 (NaCl) 诱导的胁迫中观察到更高的累加效应，这表明盐胁迫选择在幼苗期会更有效和有用。候选基因和样品表型的相对表达分析表明CCACVL1_12635和CCACVL1_15402和CCACVL1_23216基因分别是耐盐和耐旱候选基因，在胱抑素和钾离子转运蛋白1（KT1）途径中起作用。