Over the past two decades, it has become clear that the multiscale spatial and temporal organization of the genome has important implications for nuclear function. This review centers on insights gained from recent advances in light microscopy on our understanding of transcription. We discuss spatial and temporal aspects that shape nuclear order and their consequences on regulatory components, focusing on genomic scales most relevant to function. The emerging picture is that spatiotemporal constraints increase the complexity in transcriptional regulation, highlighting new challenges, such as uncertainty about how information travels from molecular factors through the genome and space to generate a functional output.

中文翻译：

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空间和时间对基因组功能输出的影响


在过去的二十年里，人们已经清楚地认识到基因组的多尺度空间和时间组织对核功能具有重要影响。这篇综述的重点是从光学显微镜的最新进展中获得的关于我们对转录的理解的见解。我们讨论了塑造核秩序的空间和时间方面及其对调控成分的影响，重点关注与功能最相关的基因组规模。新出现的情况是，时空限制增加了转录调控的复杂性，凸显了新的挑战，例如信息如何从分子因子通过基因组和空间传递以产生功能输出的不确定性。