This review summarizes the flavonoids isolated from three genera, namely, Aconitum, Delphinium, and Consolida, belonging to tribe Delphineae in the Ranunculaceae family for the first time. A total of 104 distinct flavonoid components, including 85 flavonols, 13 anthocyanins, four flavones, and two neoflavones, have been isolated from 44 members of tribe Delphineae. Flavonols account for the largest proportion and can be regarded as the dominant group of flavonoids in this tribe. Of the 104 isolated flavonoids, 55 are novel, indicating the high chemical diversity among the flavonoid constituents of Delphineae plants. Flavonoids in Delphineae plants exhibit chemotaxonomic significance, characterizing certain Delphineae species well. Flavonol glycosides, as the major flavonoid constituents in the investigated Delphineae species, could also serve as valuable chemotaxonomic markers in addition to diterpenoid alkaloids for the identification of Delphineae species.