DNA methylation is the most important epigenetic modification involved in many essential biological processes. MET1 is one of DNA methyltransferases that affect the level of methylation in the entire genome. To explore the effect of MET1 gene silencing on gene expression profile of Chrysanthemum × morifolium 'Zijingling'. The stem section and leaves at the young stage were taken for transcriptome sequencing. MET1-_RNAi leaves had 8 differentially expressed genes while 156 differentially expressed genes were observed in MET1-_RNAi stem compared with control leaves and stem. These genes encode many key proteins in plant biological processes, such as transcription factors, signal transduction mechanisms, secondary metabolite synthesis, transport and catabolism and interaction. In general, 34.58% of the differentially expressed genes in leaves and stems were affected by the reduction of the MET1 gene. The differentially expressed genes in stem and leaves of transgenic plants went through significant changes. We found adequate amount of candidate genes associated with flowering, however, the number of genes with significant differences between transgenic and control lines was not too high. Several flowering related genes were screened out for gene expression verification and all of them were obseved as consistent with transcriptome data. These candidate genes may play important role in flowering variation of chrysanthemum. This study reveals the mechanism of CmMET1 interference on the growth and development of chrysanthemum at the transcriptional level, which provides the basis for further research on the epigenetic regulation mechanism in flower induction and development.

DNA甲基化是涉及许多基本生物学过程的最重要的表观遗传修饰。MET1是影响整个基因组甲基化水平的DNA甲基转移酶之一。探讨MET1基因沉默对菊花'紫荆灵'基因表达谱的 影响 。取幼龄期的茎段和叶片进行转录组测序。MET1 - _{RNAi叶片有 8 个差异表达基因，而在}MET1 - _RNAi中观察到 156 个差异表达基因茎与对照叶和茎相比。这些基因编码植物生物学过程中的许多关键蛋白，如转录因子、信号转导机制、次生代谢产物的合成、转运和分解代谢以及相互作用。一般来说，叶和茎中34.58%的差异表达基因受到MET1减少的影响基因。转基因植物茎叶中的差异表达基因发生了显着变化。我们发现了与开花相关的足够数量的候选基因，然而，转基因和对照品系之间具有显着差异的基因数量并不太高。筛选出几个开花相关基因进行基因表达验证，所有这些基因都与转录组数据一致。这些候选基因可能在菊花开花变异中发挥重要作用。本研究从转录水平揭示了CmMET1干扰菊花生长发育的机制，为进一步研究花诱导发育的表观遗传调控机制提供依据。