Abstract

Thyroid hormones physiologically regulate the semen quality, by altering testosterone concentrations, and different seminal parameters, as well as sperm motility, viability and semen volume. Serum thyroxine (T₄), free thyroxine (fT₄), thyroid-stimulating hormone (TSH) and testosterone (T) concentrations were measured in 26 adult Labrador Retriever dogs (n. 20 normospermic and n. 6 azoospermic groups), aged 3 ± 0.5 years old, to determine their specific references, by taking into account the related conventional and kinematic sperm parameters and clinical ultrasound evaluations. The mean values of T₄, fT₄, TSH and T concentrations, as soon as those of sperm parameters and clinical evaluations of normospermic group were in line with dog’s physiological range of literature data. Normospermic group showed significant correlations between T₄ and T (r = −0.681; p < .01), sperm progressive motility (%) (r = −0.623; p < .01), and sperm non-progressive motility (%) (r = 0.625; p < .02). The azoospermic group showed higher T₄, fT₄, TSH and lower T concentrations, compared to normospermic group, and a positive correlation between T₄ and T (r = 0.8548; p < .046). The use of breed-specific hormonal ranges and sperm parameters will improve the knowledge of their interaction in Labrador Retriever dogs, adding a new segment of scientific literature.

• Highlights

• Thyroxine (T₄), free thyroxine (fT₄), thyroid stimulating hormone (TSH) and testosterone (T) concentrations were measured in male dogs.

• Conventional and kinematic sperm parameters were also evaluated.

• Dogs were divided in 20 normospermic and n. 6 azoospermic Labrador Retrivers.

• Azoospermic group showed higher values of T₄, fT₄, TSH and lower values of T compared to normospermic

• Use of breed-specific hormonal ranges and sperm parameters will improve the knowledge of scientific literature.

摘要

甲状腺激素通过改变睾酮浓度和不同的精液参数，以及精子活力、活力和精液量，在生理上调节精液质量。在 26 只成年拉布拉多犬（n . 20 正常精子组和n 6 无精子组）中测量了血清甲状腺素 (T ₄ )、游离甲状腺素 (fT ₄ )、促甲状腺激素 (TSH) 和睾酮 (T) 浓度± 0.5 岁，通过考虑相关的常规和运动精子参数以及临床超声评估来确定其具体参考。T ₄ , fT ₄的平均值，TSH和T浓度，只要精子参数和正常精子组的临床评估与文献数据的狗的生理范围一致。正常精子组显示 T ₄和 T ( r = -0.681; p < .01)、精子进行性运动 (%) ( r = -0.623; p < .01) 和精子非进行性运动 (%) ( r  = 0.625；p < .02)。与正常精子组相比，无精子组显示出更高的 T ₄、fT ₄、TSH 和更低的 T 浓度，并且 T ₄和 T之间呈正相关（r  = 0.8548；p < .046）。使用特定品种的激素范围和精子参数将提高对拉布拉多猎犬相互作用的了解，增加科学文献的新部分。

• 强调

• 在雄性犬中测量甲状腺素 (T ₄ )、游离甲状腺素 (fT ₄ )、促甲状腺激素 (TSH) 和睾酮 (T) 浓度。

• 还评估了常规和运动精子参数。

• 狗被分为 20 正常精子和 n。6 只无精子拉布拉多寻回犬。

• 与正常精子组相比，无精子组显示出更高的 T ₄、fT ₄、TSH 值和更低的 T 值。

• 使用特定品种的激素范围和精子参数将提高科学文献的知识。