Objectives

To develop a simple pectin-degrading microorganism screening method.

Results

We developed a method utilizing the phenomenon whereby cooling an alkaline agar medium containing pectin causes the agar to become cloudy. This highly simplified method involves culturing the microorganisms on pectin-containing agar medium until colony formation is observed, and subsequent overnight cooling of the agar medium to 4 °C. Using this simple procedure, we successfully identified pectin-degrading microorganisms by observing colonies with halos on the clouded agar medium. We used alkaline pectinase and Bacillus halodurans, which is known to secrete alkaline pectinase, to establish the screening method. We demonstrated the screening of pectin-degrading microorganisms using the developed method and successfully isolated pectin-degrading microorganisms (Paenibacillus sp., Bacillus clausii, and Bacillus halodurans) from a soil sample.

Conclusions

The developed method is useful for identifying pectin-degrading microorganisms.

中文翻译：

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嗜碱条件下果胶分解微生物的简单无染色筛选方法

目标

开发一种简单的果胶降解微生物筛选方法。

结果

我们开发了一种利用这种现象的方法，即冷却含有果胶的碱性琼脂培养基会导致琼脂变得混浊。这种高度简化的方法包括在含有果胶的琼脂培养基上培养微生物，直到观察到菌落形成，然后将琼脂培养基过夜冷却至 4°C。使用这个简单的程序，我们通过观察云琼脂培养基上带有光环的菌落成功地鉴定了果胶降解微生物。我们使用碱性果胶酶和已知能分泌碱性果胶酶的嗜盐芽孢杆菌来建立筛选方法。我们展示了使用开发的方法筛选果胶降解微生物并成功分离果胶降解微生物（类芽孢杆菌，Bacillus clausii和Bacillus halodurans ) 来自土壤样品。

结论

开发的方法可用于鉴定果胶降解微生物。