Notch signaling in the dynamics of perivascular stem cells and their niches,STEM CELLS Translational Medicine

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Notch signaling in the dynamics of perivascular stem cells and their niches
STEM CELLS Translational Medicine ( IF 5.4 ) Pub Date : 2021-07-06 , DOI: 10.1002/sctm.21-0086
Pierfrancesco Pagella ₁ , Laura de Vargas Roditi _{2,

3} , Bernd Stadlinger ₄ , Andreas E Moor _{2,

3} , Thimios A Mitsiadis ₁

Affiliation

The Notch signaling pathway is a fundamental regulator of cell fate determination in homeostasis and regeneration. In this work, we aimed to determine how Notch signaling mediates the interactions between perivascular stem cells and their niches in human dental mesenchymal tissues, both in homeostatic and regenerative conditions. By single cell RNA sequencing analysis, we showed that perivascular cells across the dental pulp and periodontal human tissues all express NOTCH3, and that these cells are important for the response to traumatic injuries in vivo in a transgenic mouse model. We further showed that the behavior of perivascular NOTCH3-expressing stem cells could be modulated by cellular and molecular cues deriving from their microenvironments. Taken together, the present studies, reinforced by single-cell analysis, reveal the pivotal importance of Notch signaling in the crosstalk between perivascular stem cells and their niches in tissue homeostasis and regeneration.

中文翻译：

血管周围干细胞及其生态位动力学中的缺口信号

Notch 信号通路是稳态和再生中细胞命运决定的基本调节因子。在这项工作中，我们旨在确定在稳态和再生条件下，Notch 信号如何介导血管周围干细胞与其在人类牙齿间充质组织中的生态位之间的相互作用。通过单细胞 RNA 测序分析，我们发现跨过牙髓和牙周人体组织的血管周围细胞都表达NOTCH3，并且这些细胞对于转基因小鼠模型中对体内创伤性损伤的反应很重要。我们进一步证明了血管周围NOTCH3的行为-表达干细胞可以通过源自其微环境的细胞和分子信号进行调节。总之，目前的研究，通过单细胞分析，揭示了 Notch 信号在血管周围干细胞与其在组织稳态和再生中的生态位之间的串扰中的关键重要性。

更新日期：2021-07-06

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