ABSTRACT

Histone modification influences gene expression. Among histone modifications, H3K27me3 is associated with downregulation of nearby genes via chromatin compaction. In Arabidopsis thaliana, a subset of JUMONJI C DOMAIN-CONTAINING PROTEIN (JMJ) proteins play a critical role in removal of H3K27me3 during plant development or in response to environmental cues. However, the regulation of H3K27me3 demethylase gene expression is not yet fully characterized. In this study, we computationally characterized the expression patterns of JMJ H3K27me3 demethylase genes using public transcriptome datasets created across plant development and after various environmental cues. Consistent with the available transcriptome datasets, GUS staining validated that JMJ30 was highly expressed in the L1 layer of the shoot apical meristem. Furthermore, expression data for panel of five H3K27me3 demethylase genes revealed JMJ30 to be the most highly affected by abiotic and biotic stress. In addition, JMJ30 expression was variable between Arabidopsis thaliana accessions. Finally, the expression of a JMJ30 orthologue from the related species Arabidopsis halleri, AhgJMJ30, fluctuated under field conditions. Taken together, our results suggest that transcriptional changes of H3K27me3 demethylase genes may play key roles in development and environmental responses.

摘要

组蛋白修饰影响基因表达。在组蛋白修饰中，H3K27me3 通过染色质压缩与附近基因的下调有关。在拟南芥中，JUMONJI C 域含蛋白 (JMJ) 蛋白的一个子集在植物发育过程中或响应环境线索时在去除 H3K27me3 中起关键作用。然而，H3K27me3 去甲基化酶基因表达的调节尚未完全表征。在这项研究中，我们使用跨植物发育和各种环境线索创建的公共转录组数据集计算表征 JMJ H3K27me3 去甲基化酶基因的表达模式。与可用的转录组数据集一致，GUS 染色验证了JMJ30在茎尖分生组织的 L1 层中高度表达。此外，五个 H3K27me3 去甲基化酶基因组的表达数据显示JMJ30受非生物和生物胁迫的影响最大。此外，JMJ30表达在拟南芥种质之间是可变的。最后，来自相关物种拟南芥的JMJ30直系同源物 AhgJMJ30 的表达在田间条件下波动。总之，我们的结果表明 H3K27me3 去甲基化酶基因的转录变化可能在发育和环境反应中起关键作用。