Chitinase-producing Trichoderma species have been recognized long ago against the phytopathogenic fungi. In this study, we evaluated the production of chitinase enzyme for seventeen isolates of Trichoderma isolated from onion growing districts of Punjab and assessed their bio-efficacy against damping-off in onion. In vitro, these Trichoderma isolates were screened for their antagonistic activity against the damping-off pathogen Fusarium oxysporum f.sp. cepae by dual culture assay. These isolates were also screened for their chitinase enzyme activity; it was found that isolates T5 and T8 are showing higher antagonistic activity on Fusarium oxysporum f.sp. cepae and also produced large amounts of chitinase enzymes in the presence of commercial colloidal chitin. The selected chitinolytic isolates were used in field studies to confirm the feasibility of their biological control efficacy against onion damping-off. In the field experiment, the seed+soil treatment of chitinolytic isolate (T8) showed a critical decrease of damping-off in onion by 88.75% over control.

很久以前就已经认识到产生几丁质酶的木霉属可以对抗植物病原真菌。在这项研究中，我们评估了从旁遮普省洋葱种植区分离的17 株木霉属分离株的几丁质酶的产量，并评估了它们对洋葱猝倒病的生物效力。在体外，筛选了这些木霉属分离株对猝灭病原体尖孢镰刀菌f.sp.的拮抗活性。cepae通过双重培养测定。还筛选了这些分离株的几丁质酶活性；我们发现菌株T5和T8展示在较高的拮抗活性尖镰孢病菌cepae并且还在商业胶体几丁质存在下产生大量几丁质酶。选定的几丁质分离物用于田间研究，以确认它们对洋葱猝倒病的生物防治功效的可行性。在田间试验中，几丁质分离物 (T8) 的种子 + 土壤处理显示洋葱的猝倒率比对照降低了 88.75%。