当前位置: X-MOL 学术ACS Food Sci. Technol. › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Immobilized Reporter Phage on Electrospun Polymer Fibers for Improved Capture and Detection of Escherichia coli O157:H7
ACS Food Science & Technology ( IF 2.6 ) Pub Date : 2021-07-06 , DOI: 10.1021/acsfoodscitech.1c00101
Sim Yee Chen 1 , Melissa Harrison 1 , Eng Khoon Ng 2 , Dominic Sauvageau 1 , Anastasia Elias 1
Affiliation  

Escherichia coli O157:H7 is a foodborne Shiga-toxin-producing bacterium that leads to millions of cases of illness every year. The development of a portable biosensor that rapidly detects this pathogen can greatly reduce both the time required to identify the source of contamination within the food production chain and the scale of outbreaks. Here we propose a swab for the detection of E. coli O157:H7 made from electrospun poly-3-hydroxybutyrate (PHB) on which the reporter bacteriophage (phage), PP01—engineered to express the bioluminescent protein Nanoluc upon infection of the pathogen—has been immobilized. In the development of the material, the properties of phage-immobilized electrospun porous, nonwoven PHB mats were compared to those of phage-immobilized flat PHB films produced by solvent casting. Dynamics of E. coli infections initiated with the phage-immobilized materials showed the electrospun mats had the highest infectivity, indicative of higher phage surface density (confirmed by microscopy) and activity—two important properties for the sensitivity and response time of biosensors. In sample tests, successful detection of E. coli O157:H7 cells in both milk and Tryptic Soy Broth at concentrations of 105 CFU/mL within 1 h and 101 CFU/mL within 3 h demonstrates the promising potential of the system as a food safety assessment tool.

中文翻译:

电纺聚合物纤维上的固定化报告噬菌体用于改进大肠杆菌 O157:H7 的捕获和检测

大肠杆菌O157:H7 是一种食源性志贺毒素产生细菌,每年导致数百万例疾病。快速检测这种病原体的便携式生物传感器的开发可以大大减少确定食品生产链中污染源所需的时间和爆发的规模。在这里,我们提出了一种用于检测大肠杆菌的拭子O157:H7 由电纺聚 3-羟基丁酸酯 (PHB) 制成,其上固定了报道噬菌体(噬菌体)PP01——设计用于在病原体感染时表达生物发光蛋白 Nanoluc——已被固定。在该材料的开发过程中,将噬菌体固定化电纺多孔非织造 PHB 垫的特性与通过溶剂浇铸生产的噬菌体固定化平面 PHB 薄膜的特性进行了比较。用噬菌体固定材料引发的大肠杆菌感染动力学显示,电纺垫具有最高的感染性,表明噬菌体表面密度(通过显微镜确认)和活性更高——这是生物传感器灵敏度和响应时间的两个重要特性。在样品测试中,成功检测到大肠杆菌牛奶和胰蛋白酶大豆肉汤中的 O157:H7 细胞在1 小时内浓度为 10 5 CFU/mL,在 3 小时内浓度为 10 1 CFU/mL,这证明了该系统作为食品安全评估工具的潜力。
更新日期:2021-07-16
down
wechat
bug