This study concentrates on the isolation of bio colour producing bacteria from goat droppings and application of its colour pigment in the textile dyeing process. The isolated strain is subjected to biochemical as well as molecular characterization and is identified as Pseudomonas aeruginosa using thin layer chromatography. An optimum condition for production of pigment ‘Pyocyanin (blue-green)’ has been studied by one-factor at a time approach. The pigment (Pyocyanin) is extracted using chloroform, and 0.2N hydrochloric acid. The application of this extracted bio color as coloring agent on polyester fabric has also been studied, and it is found that the color retained on the fabric is yellow, which is different from the extracted pigment colour. Color quality evaluation of dyed polyester fabric has been performed with references to international standard protocols. The color producing is found to be 1-hydroxyphenazine, obtained by the hydrolysis of Pyocyanin at high temperature (130oC). This 1-hydroxyphenazine imparts the yellow shade to polyester fabric on dyeing.

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生产细菌色素的铜绿假单胞菌的分离及其在合成针织面料上的应用

本研究集中于从山羊粪便中分离生色菌及其有色颜料在纺织品染色过程中的应用。对分离的菌株进行生化和分子表征，并使用薄层色谱法将其鉴定为铜绿假单胞菌。已经通过一次一个因素的方法研究了生产色素“绿脓素（蓝绿色）”的最佳条件。使用氯仿和 0.2N 盐酸提取色素（绿脓素）。还研究了这种提取的生物色素作为着色剂在涤纶织物上的应用，发现保留在织物上的颜色是黄色，与提取的颜料颜色不同。已参照国际标准协议对染色涤纶织物进行颜色质量评估。发现产生颜色的是 1-羟基吩嗪，它是通过在高温 (130oC) 下水解绿脓菌素获得的。这种 1-羟基吩嗪在染色时赋予聚酯织物黄色色调。