Glyphosate [N-(phosphonomethyl)glycine] is the active ingredient in widely used broad-spectrum herbicides. Even though the toxicity mechanism of this herbicide in vertebrates is poorly understood, evidence suggests that glyphosate is an endocrine disruptor capable of producing morphological anomalies as well as cardiotoxic and neurotoxic effects. We used the zebrafish model to assess the effects of early life glyphosate exposure on the development of cartilage and bone tissues and organismal responses. We found functional alterations, including a reduction in the cardiac rate, significant changes in the spontaneous tail movement pattern, and defects in craniofacial development. These effects were concomitant with alterations in the level of the estrogen receptor alpha osteopontin and bone sialoprotein. We also found that embryos exposed to glyphosate presented spine deformities as adults. These developmental alterations are likely induced by changes in protein levels related to bone and cartilage formation.

草甘膦[ N- (膦酰甲基)甘氨酸]是广泛使用的广谱除草剂的活性成分。尽管人们对这种除草剂对脊椎动物的毒性机制知之甚少，但有证据表明，草甘膦是一种内分泌干扰物，能够产生形态异常以及心脏毒性和神经毒性作用。我们使用斑马鱼模型来评估生命早期接触草甘膦对软骨和骨组织发育以及机体反应的影响。我们发现了功能改变，包括心率降低、自发尾部运动模式的显着变化以及颅面发育缺陷。这些效应伴随着雌激素受体α骨桥蛋白和骨唾液蛋白水平的改变。我们还发现，暴露于草甘膦的胚胎成年后会出现脊柱畸形。这些发育变化可能是由与骨和软骨形成相关的蛋白质水平的变化引起的。