Phosphate (Pi) fertilizer is applied in huge amount due to the poor absorption of Pi by crops especially in acidic tropical soil. As the major global source of oil and fats, the mechanism of Pi transport in oil palm needs to be studied to maintain good productivity for sustainable palm oil production. Ten Elaeis guineensis Phosphate Transporter1 (EgPHT1) and three Elaeis guineensis Phosphate Starvation Response (EgPHR) genes were identified in this study. All EgPHT1 proteins contain GGDYPLSATIxSE, the signature sequence of PHT1. All EgPHR have MYB-binding domain and coiled-coil domain characteristic of PHR at their C-terminal regions and one unique SOG2 domain for EgPHR1. The expression of four of the EgPHT1 and two of the EgPHR under low Pi (LP) and Pi starvation (-P) was studied by real-time quantitative PCR (qPCR). All genes showed enhanced expression in roots at −P compared to +P but no detectable change in the leaves. The expression profile of EgPHR2 which showed significant upregulation at LP compared to +P and further increase at −P correlated with EgPHT1;4 and EgPHT1;7 that possess P1BS motif in their promoter sequences, the binding site for PHR. PHR2, as a potential early transcriptional regulator for phosphate starvation was proven to be nuclear localized by subcellular localization experiment. Altogether, this study suggests all four analyzed EgPHT1 and two EgPHR play critical role in responding to Pi deprivation in oil palm. EgPHT1;4 and EgPHT1;7 which possess the P1BS motif are potentially upregulated by EgPHR2 as an early response mechanism against phosphate starvation.

由于作物对磷的吸收较差，特别是在酸性热带土壤中，磷肥被大量施用。作为全球主要的油脂来源，需要研究油棕中 Pi 的传输机制，以保持棕榈油可持续生产的良好生产力。本研究鉴定了10个Elaeis guineensis Phosphate Transporter1 ( EgPHT1 )和3个Elaeis guineensis磷酸盐饥饿反应( EgPHR )基因。所有的 EgPHT1 蛋白都包含 GGDYPLSATIxSE，这是 PHT1 的特征序列。所有 EgPHR 在其 C 端区域都具有 PHR 特有的 MYB 结合域和卷曲螺旋域，以及一个独特的 EgPHR1 SOG2 域。EgPHT1的四种表达并且通过实时定量 PCR (qPCR) 研究了低 Pi (LP) 和 Pi 饥饿 (-P) 下的两个EgPHR。与+P 相比，所有基因在-P 下在根中的表达增强，但在叶子中没有可检测到的变化。EgPHR2 的表达谱显示与 +P 相比在 LP 处显着上调，并在 -P 处进一步增加与EgPHT1;4和EgPHT1;7 相关，它们的启动子序列中具有 P1BS 基序，PHR 的结合位点。PHR2 作为磷酸盐饥饿的潜在早期转录调节因子，已被亚细胞定位实验证明是核定位的。总之，这项研究表明所有四个分析的EgPHT1和两个EgPHR在应对油棕中的缺磷方面发挥关键作用。拥有 P1BS 基序的EgPHT1;4和EgPHT1;7可能被EgPHR2上调，作为对抗磷酸盐饥饿的早期反应机制。