A self-labeling protein based on the small ultra-red fluorescent protein, smURFP,RSC Chemical Biology

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A self-labeling protein based on the small ultra-red fluorescent protein, smURFP
RSC Chemical Biology ( IF 4.2 ) Pub Date : 2021-06-29 , DOI: 10.1039/d1cb00127b
John-Hanson Machado ₁ , Richard Ting _{2,

3} , John Y Lin ₄ , Erik A Rodriguez ₁

Affiliation

Self-labeling proteins have revolutionized super-resolution and sensor imaging. Tags recognize a bioorthogonal substrate for covalent attachment. We show the small Ultra-Red Fluorescent Protein (smURFP) is a self-labeling protein. The substrate is fluorogenic, fluoresces when attached, and quenches fluorescent cargo. The smURFP-tag has novel properties for tool development.

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