Quercetin is a flavonoid compound with a variety of biological properties that is widely distributed throughout the plant kingdom. Studies have found that quercetin has anti-inflammatory, antioxidant, and liver-protective effects, while thioacetamide (TAA) can cause inflammation and liver damage in zebrafish larvae. The purpose of this study was to evaluate whether quercetin can prevent TAA-induced inflammation and liver damage in zebrafish larvae and to investigate the molecular mechanisms involved. Zebrafish Tg transgenic lines were used as the experimental animals. Behavioral, oxidative stress level, proliferative antigen chromogenic antibody, and western blot analyses were carried out on zebrafish larvae in the control group and groups treated with TAA and 12 μM quercetin. The results indicated that quercetin promoted the development of zebrafish larvae damaged by TAA, exhibited antioxidant and anti-inflammatory properties, and promoted cell proliferation. Quercetin reduced the expression of p53 protein in zebrafish larvae injured by TAA, resulting in decreased levels of Bax and increased levels of Bcl-2. The findings suggested quercetin has antiapoptotic action. Quercetin reduced the expression of DKK1 and DKK2 genes related to the Wnt signaling pathway in zebrafish larvae damaged by TAA and increased the expression of Lef1 and wnt2bb. Quercetin may regulate the development of zebrafish larvae damaged by TAA through the Wnt signaling pathway. This study provides the scientific basis for the development and utilization of quercetin and the development of new related drugs.

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硫代乙酰胺的毒性和槲皮素对斑马鱼（Danio rerio）幼虫的保护作用

槲皮素是一种黄酮类化合物，具有多种生物学特性，广泛分布于整个植物界。研究发现，槲皮素具有抗炎、抗氧化和保肝作用，而硫代乙酰胺（TAA）可引起斑马鱼幼虫的炎症和肝损伤。本研究的目的是评估槲皮素是否可以预防斑马鱼幼虫 TAA 诱导的炎症和肝损伤，并研究相关的分子机制。斑马鱼 Tg 转基因系用作实验动物。对对照组和用 TAA 和 12 μM 槲皮素处理的组中的斑马鱼幼虫进行行为、氧化应激水平、增殖抗原显色抗体和蛋白质印迹分析。结果表明，槲皮素促进了被TAA损伤的斑马鱼幼虫的发育，表现出抗氧化和抗炎特性，促进细胞增殖。槲皮素降低了受 TAA 损伤的斑马鱼幼虫中 p53 蛋白的表达，导致 Bax 水平降低和 Bcl-2 水平升高。研究结果表明槲皮素具有抗细胞凋亡作用。槲皮素降低了DKK1和DKK2基因相关的Wnt信号传导途径中由TAA损坏斑马鱼幼体和增加的表达LEF1和wnt2bb。槲皮素可能通过 Wnt 信号通路调节被 TAA 损伤的斑马鱼幼虫的发育。该研究为槲皮素的开发利用和相关新药的开发提供了科学依据。