We investigated the use of entomopathogenic nematodes to biologically control Bactrocera dorsalis in mango orchards. One isolate of Steinernema kandii (Thui) and two of Heterorhabditis taysearae (Hessa1 and Korobororou F4) were studied for their invasion time and virulence to third instar larvae of B. dorsalis in laboratory and semi field tests, respectively. In addition, the persistence of the same nematode isolates in soil under field conditions was tested. Results showed that all three nematode isolates could penetrate insect larvae after 2 h of exposure time. Furthermore, under semi field conditions, insect mortality was significantly different among EPN application times. The three nematode isolates were highly pathogenic to B. dorsalis with H. taysearae Hessa1 being the most virulent (70.84% ± 10.46 [SEM] mortality) when EPNs were applied three days before insect introduction in the experimental pots. Moreover, Steinernema kandii persisted in soil up to 32 weeks after nematode application whereas both H. taysearae isolates persisted 30 weeks post application in the mango orchard. In general, four weeks upon nematode application, the density of infective juveniles decreased considerably and remained variable the following sampling dates.

我们调查了使用昆虫病原线虫对芒果园中的Bactrocera dorsalis进行生物控制。Steinernema kandii (Thui)分离株和Heterorhabditis taysearae (Hessa1 和 Korobororou F4)分离株分别在实验室和半田间试验中研究了它们的入侵时间和对B. dorsalis三龄幼虫的毒力。此外，还测试了田间条件下土壤中相同线虫分离株的持久性。结果表明，暴露 2 小时后，所有三种线虫分离株都能穿透昆虫幼虫。此外，在半田条件下，不同 EPN 施用时间的昆虫死亡率存在显着差异。三种线虫分离株对线虫具有高致病性B. dorsalis与H. taysearae Hessa1 是最毒力的（70.84% ± 10.46 [SEM] 死亡率），当在实验盆中昆虫引入前三天应用 EPN 时。此外，施泰因线虫在土壤中持续存在长达 32 周的线虫施用后，而两种H. taysearae 分离物在芒果园中持续存在 30 周。一般而言，在施用线虫 4 周后，感染幼虫的密度显着降低，并且在接下来的采样日期保持可变。