The sustainability in polyhydroxyalkanoates (PHA) production is drawing increasing attention as the effort to increase the economic feasibility for commercialization pursues. Oleic acid is widely preferred by bacteria but its employment for PHA production makes sustainability rather dubious. This study showed promising poly(3-hydroxybutyrate-co-3-hydroxyvalerate) [P(3HB-co-3HV)] content of 68 wt % by lipase genes-harbouring Cupriavidus malaysiensis USMAA2-4 transformant from palm olein and 1-pentanol. High oleic acid content and low oil saturation caused palm olein to outperform crude palm oil, crude palm kernel oil and soybean oil due to its preference for oleic acid shown by previous screening. The transformant showed 8-fold and 40-fold higher lipase activity compared to C. necator H16 and its wild-type respectively. The transformant was unaffected by Co²⁺ but the growth of C. necator H16 was inversely proportional to Co²⁺ concentration and the employment of 1-pentanol also ceased its growth and PHA accumulation. Although the inhibitory effect of Fe²⁺, Cu²⁺ and Zn²⁺ at high molarity on LipA decreased PHA content of C. malaysiensis USMAA2-4 transformant by 23–24 wt %, the lipase activity was restorable with high molarity of Ca²⁺, thus resulted in higher PHA content. The transformant enabled the employment of low-cost 1-pentanol as the precursor for cost-effective PHA production and its preference for palm olein contributed to higher sustainability.

随着努力提高商业化的经济可行性，聚羟基链烷酸酯 (PHA) 生产的可持续性正引起越来越多的关注。细菌广泛偏爱油酸，但将其用于 PHA 生产使得可持续性相当可疑。这项研究显示有希望的聚（3-羟基丁酸酯共-3-羟基戊酸酯）[P（3HB-共-3HV）]的68重量％的脂肪酶的基因，携带内容钩虫贪铜的马来西亚来自棕榈油精和 1-戊醇的 USMAA2-4 转化体。高油酸含量和低油饱和度导致棕榈油精优于毛棕榈油、毛棕榈仁油和豆油，因为其对先前筛选显示的油酸的偏爱。与C. necator H16 及其野生型相比，转化体分别显示出高8 倍和40 倍的脂肪酶活性。转化体不受Co ^{2+ 的}影响，但C. necator H16的生长与Co ²⁺浓度成反比，使用1-戊醇也停止了其生长和PHA 积累。Fe ²⁺、Cu ²⁺和Zn ²⁺的抑制作用虽然在 LipA 上的高摩尔浓度将C. malaysiensis USMAA2-4 转化体的PHA 含量降低了 23-24 wt%，脂肪酶活性可通过高摩尔浓度的 Ca ²⁺恢复，从而导致更高的 PHA 含量。该转化体能够使用低成本的 1-戊醇作为具有成本效益的 PHA 生产的前体，其对棕榈油精的偏好有助于提高可持续性。