Amphibian thrombocyte-derived extracellular vesicles, including microRNAs, induce angiogenesis-related genes in endothelial cells,Genes to Cells

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Amphibian thrombocyte-derived extracellular vesicles, including microRNAs, induce angiogenesis-related genes in endothelial cells
Genes to Cells ( IF 1.3 ) Pub Date : 2021-07-05 , DOI: 10.1111/gtc.12882
Kenkichi Sugimoto ₁ , Kayano Toume ₁

Affiliation

Thrombocytes circulate in the blood of nonmammalian vertebrates and are involved in hemostasis; however, many detailed characteristics of thrombocytes remain unclear. Recently, we established an amphibian thrombocyte cell line. Here, we report the finding that thrombocytes produce integrin alpha IIb (CD41)-positive extracellular vesicles (EVs), which include microRNAs (miRs). Flow cytometric analysis showed the expression of CD41⁺ and phosphatidylserine on the surface of EVs. Nanotracking analysis showed that these CD41⁺ EVs were approximately 100 nm in diameter. As CD41⁺ EVs were also observed from African clawed frogs, the production of CD41⁺ EVs might be common to amphibians. Microarray analysis showed that the CD41⁺ EVs contain many kinds of miRs. These CD41⁺ EVs were phagocytosed by endothelial cells and macrophages. qPCR analysis showed that many angiogenesis-related genes were up-regulated in CD41⁺ EV-treated endothelial cells. Over-expression of some miRs in the CD41⁺ EVs increased the proliferation of endothelial cells. These results indicated that thrombocytes produced CD41⁺ EVs, including miRs, that were received by endothelial cells to induce the expression of angiogenesis-related genes. These results indicated that the CD41⁺ EVs produced from thrombocytes act as signaling molecules to repair damaged blood vessels.

中文翻译：

两栖类血小板衍生的细胞外囊泡，包括 microRNA，在内皮细胞中诱导血管生成相关基因

血小板在非哺乳动物脊椎动物的血液中循环并参与止血；然而，血小板的许多详细特征仍不清楚。最近，我们建立了一个两栖类血小板细胞系。在这里，我们报告了血小板产生整合素 α IIb (CD41) 阳性细胞外囊泡 (EVs) 的发现，其中包括 microRNAs (miRs)。流式细胞仪分析显示EV 表面CD41 ^{+和磷脂酰丝氨酸的表达。}纳米跟踪分析表明，这些 CD41 ⁺ EV 的直径约为 100 nm。由于在非洲爪蛙中也观察到CD41 ^{+ EV，因此 CD41}⁺ EV 的产生可能在两栖动物中很常见。微阵列分析表明，CD41 ⁺EV 包含多种 miR。这些 CD41 ⁺ EV 被内皮细胞和巨噬细胞吞噬。qPCR 分析表明，许多血管生成相关基因在 CD41 ⁺ EV 处理的内皮细胞中上调。^{CD41 +} EVs 中一些 miRs 的过度表达增加了内皮细胞的增殖。这些结果表明，血小板产生 CD41 ⁺ EVs，包括 miRs，被内皮细胞接收以诱导血管生成相关基因的表达。这些结果表明，血小板产生的 CD41 ⁺ EVs 作为信号分子来修复受损的血管。

更新日期：2021-07-05

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