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Lethal temperature and toxicity of ammonia in juveniles of Curimbatá (Prochilodus lineatus)
Aquaculture ( IF 3.9 ) Pub Date : 2021-07-05 , DOI: 10.1016/j.aquaculture.2021.737138
Tais Inês Zuffo 1 , Emerson Giuliani Durigon 2 , Monique Berticelli Morselli 1 , Fernanda Picoli 3 , Sidinei Folmann 4 , José Fernando Kinas 5 , Thaiza Savaris 6 , Aline Zampar 7 , Diogo Luiz De Alcantara Lopes 7
Affiliation  

The maintenance of quality parameters in fish farming is fundamental to the success of production systems. Ammonia is toxic when present in high concentrations, causing inadequate development, loss of growth, and mortality in fish. Temperature can also cause biochemical and physiological changes in organisms and cause mortality. Curimbatá Prochilodus spp. have the potential to be used in fish farming in polycultures in Brazil. However, few studies have been carried out regarding adequate water quality parameters for the production of Curimbatá. Thus, the present study determined the lower and upper lethal temperatures (LT50), as well as the lethal concentration (LC50), and sublethal and toxic effects of ammonia on juveniles of Curimbatá Prochilodus lineatus through tests of acute exposure for 96 h. In the lethal temperature test, the fish were subjected to treatments of 10 °C, 15 °C, 20 °C, 25 °C, 30 °C, 35 °C, and 40 °C. Temperatures of 10 °C and 40 °C were lethal at 36 and 24 h, respectively. The lower and upper LT50 were 13.8 ± 1.14 and 39.7 ± 1.59 °C at 36 and 12 h, respectively. For the determination of lethal and sublethal concentrations of ammonia (NH3), the fish were subjected to treatments 0.00, 0.75, 1.14, 1.47, 1.82, and 2.21 (mg/L NH3). The LC50 determined for 96 h was 0.62 mg/L and the LC10 for 24 h was 0.93 mg/L. In addition, histopathological evaluation of the gills was adequate to observe the effects of acute ammonia concentrations. Ammonia concentrations above 0.75 mg/L caused irreversible histological changes. The results of the study indicates that the ideal temperature range for breeding Curimbatá is 25 °C to 30 °C, since this variation did not influence fish survival. In addition, Curimbatá should be grown in water with concentrations below 0.75 mg/L of ammonia.



中文翻译:

Curimbatá ( Prochilodus lineatus )幼鱼中氨的致死温度和毒性

鱼类养殖质量参数的保持是生产系统成功的基础。氨以高浓度存在时是有毒的,会导致鱼类发育不充分、生长丧失和死亡。温度还会引起生物体的生化和生理变化并导致死亡。Curimbatá Prochilodus spp。有可能用于巴西混养的鱼类养殖。然而,关于库林巴塔生产的适当水质参数的研究很少。因此,本研究确定了较低和较高的致死温度 (LT 50 ),以及致死浓度 (LC 50 ),以及氨对库林巴塔幼鱼的亚致死和毒性作用Prochilodus lineatus通过 96 小时的急性暴露测试。在致死温度试验中,对鱼进行了10℃、15℃、20℃、25℃、30℃、35℃和40℃的处理。10 °C 和 40 °C 的温度分别在 36 和 24 小时时致死。下和上 LT 50在 36 和 12 小时时分别为 13.8 ± 1.14 和 39.7 ± 1.59 °C。为了测定氨 (NH 3 )的致死和亚致死浓度,对鱼进行了 0.00、0.75、1.14、1.47、1.82 和 2.21 (mg/L NH 3 ) 的处理。96 小时测定的 LC 50为 0.62 mg/L,LC 1024 小时为 0.93 毫克/升。此外,鳃的组织病理学评估足以观察急性氨浓度的影响。氨浓度高于 0.75 mg/L 会导致不可逆的组织学变化。研究结果表明,库林巴塔繁殖的理想温度范围是 25 °C 至 30 °C,因为这种变化不会影响鱼类的生存。此外,库林巴塔应在氨浓度低于 0.75 毫克/升的水中生长。

更新日期:2021-07-16
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