Contribution of a DNA Nick to DNA Bendability Depending on the Bending Force,Bulletin of the Korean Chemical Society

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Contribution of a DNA Nick to DNA Bendability Depending on the Bending Force
Bulletin of the Korean Chemical Society ( IF 2.3 ) Pub Date : 2021-07-04 , DOI: 10.1002/bkcs.12351
Sanghun Yeou ₁ , Nam Ki Lee ₂

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A DNA nick plays a pivotal role in the bending and release of mechanical stress for double-stranded DNA (dsDNA). However, it remains unclear how a DNA nick modulates the bendability of dsDNA. Here, we report that a single nick in 30 base-pair dsDNA decreases the bending stiffness of dsDNA by 20%â€“30% and that the bendability of dsDNA depends on the applied bending force. To achieve this, we used D-shaped DNA nanostructures and single-molecule fluorescence resonance energy transfer for measurement of the bending angle of dsDNA. We found that a nick in dsDNA under a bending force increases the bendability of dsDNA and the bending stiffness of dsDNA decreases further as the applied bending force is increased. Our results provide a new perspective for the effect of a nick on dsDNA mechanics, and the methods introduced here will be useful for investigating various biological processes involving DNA nicks.

中文翻译：

DNA 缺口对 DNA 弯曲性的贡献取决于弯曲力

DNA 缺口在双链 DNA (dsDNA) 的弯曲和机械应力释放中起着关键作用。然而，目前尚不清楚 DNA 切口如何调节 dsDNA 的可弯曲性。在这里，我们报告了 30 碱基对 dsDNA 中的单个切口将 dsDNA 的弯曲刚度降低了 20% - 30%，并且 dsDNA 的弯曲性取决于施加的弯曲力。为此，我们使用 D 形 DNA 纳米结构和单分子荧光共振能量转移来测量 dsDNA 的弯曲角度。我们发现在弯曲力下 dsDNA 中的切口会增加 dsDNA 的弯曲性，并且随着施加的弯曲力的增加，dsDNA 的弯曲刚度进一步降低。我们的结果为缺口对 dsDNA 力学的影响提供了新的视角，

更新日期：2021-08-19

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